A5GLH6 (ISPE_SYNPW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	SynWH7803_1365

Organism

Synechococcus sp. (strain WH7803) [Complete proteome] [HAMAP]

Taxonomic identifier

32051 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechococcus

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000335764
Regions
	Nucleotide binding	97 – 107	11	ATP Potential
Sites
	Active site	12	1	By similarity
	Active site	139	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5GLH6 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 5184F7D1C8CE6F4D	FASTA	309	33,231
	10 20 30 40 50 60 MTAPVCVTAP AKINLHLEVL GLRADGFHEL AMVMQSIDLQ DQLHCENTSD GSISLRCDAS 70 80 90 100 110 120 ELSCGEDNLI VRAARLLRER SGFSELGARM HLKKHIPIGA GLAGGSSDGA AALVALNELW 130 140 150 160 170 180 GLSLDASQLE RFAAELGSDM PFCVAGGTQL CFGRGERLEP LPPLEAQLGI LLVKDPDVSV 190 200 210 220 230 240 STPWAYSECR RQRGSTYLQD EAAFEARREA LRAAQWLHPL GGEAPPLRND LQTVVEPLTP 250 260 270 280 290 300 SVRDALQCLA SLPNCLQAAM SGSGPSCFAL FHNRGEADRA KDQVQTQLDQ LGLRSWSCSL IPHGVKLMA

References

[1]	Genoscope Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WH7803.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CT971583 Genomic DNA. Translation: CAK23791.1.
RefSeq	YP_001225088.1. NC_009481.1.
3D structure databases
ProteinModelPortal	A5GLH6.
ModBase	Search...
Protein-protein interaction databases
STRING	A5GLH6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5148180.
GenomeReviews	Gene locus SynWH7803_1365 in contig CT971583_GR.
KEGG	syx:SynWH7803_1365.
PATRIC	23828870. VBISynSp43824_1396.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HBG734593.
OMA	QLCFGRG.
PhylomeDB	A5GLH6.
ProtClustDB	PRK02534.
Enzyme and pathway databases
BioCyc	SSP32051:SYNWH7803_1365-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_SYNPW

Accession

Primary (citable) accession number: A5GLH6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	June 12, 2007
Last modified:	December 14, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program