A5GJ48 (MTNC_SYNPW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Enolase-phosphatase E1
EC=3.1.3.77

Alternative name(s):
2,3-diketo-5-methylthio-1-phosphopentane phosphatase

Gene names

Name:	mtnC
Ordered Locus Names:	SynWH7803_0537

Organism

Synechococcus sp. (strain WH7803) [Complete proteome] [HAMAP]

Taxonomic identifier

32051 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechococcus

Protein attributes

Sequence length	242 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) By similarity. HAMAP MF_01681
Catalytic activity	5-(methylthio)-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate. HAMAP MF_01681
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01681
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6. HAMAP MF_01681 Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 4/6.
Subunit structure	Monomer By similarity. HAMAP MF_01681
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. MasA/mtnC family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-methionine salvage from methylthioadenosine Inferred from electronic annotation. Source: InterPro
Molecular function	2,3-diketo-5-methylthiopentyl-1-phosphate enolase activity Inferred from electronic annotation. Source: InterPro 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase activity Inferred from electronic annotation. Source: InterPro acireductone synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 242	242	Enolase-phosphatase E1 HAMAP MF_01681		PRO_0000357424

Sequences

	Sequence		Length	Mass (Da)
	A5GJ48 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: EA9831B9AC383E72	FASTA	242	26,814
	10 20 30 40 50 60 MIKAIVLDIE GTTCPVTFVS QTLFPFARRQ LSKTICSENR PSNVTAAIEE AIAEWKNDPE 70 80 90 100 110 120 SKSQALLLRA SNQNQPTSED IIHYFDHLIQ NDRKSTALKE LQGIIWEQGY AAGELQSPLY 130 140 150 160 170 180 GDVVPALNAW TQQGITLAVY SSGSVKAQQL LYAHTTDGDI TNRFSQWFDT RTGPKLNADS 190 200 210 220 230 240 YRIISQSIGL KPASILFVSD HPRECDAAAE AGMETRFCLR EGNPFSDGGD HQMIHNLGEI KL

References

[1]	Genoscope Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WH7803.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CT971583 Genomic DNA. Translation: CAK22963.1.
RefSeq	YP_001224260.1. NC_009481.1.
3D structure databases
ProteinModelPortal	A5GJ48.
ModBase	Search...
Protein-protein interaction databases
STRING	A5GJ48.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5146545.
GenomeReviews	Gene locus SynWH7803_0537 in contig CT971583_GR.
KEGG	syx:SynWH7803_0537.
PATRIC	23827163. VBISynSp43824_0548.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG4229.
HOGENOM	HBG294242.
OMA	TTDLNFI.
PhylomeDB	A5GJ48.
ProtClustDB	CLSK866737.
Enzyme and pathway databases
BioCyc	SSP32051:SYNWH7803_0537-MONOMER.
Family and domain databases
HAMAP	MF_01681. Salvage_MtnC. [Tree]
InterPro	IPR005834. Dehalogen-like_hydro. IPR023943. Enolase-phosphatase_E1. IPR010041. Enolase_ppase. IPR023214. HAD-like_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.1000. HAD-like_dom. 1 hit.
KO	K09880.
Pfam	PF00702. Hydrolase. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01691. Enolase-ppase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTNC_SYNPW

Accession

Primary (citable) accession number: A5GJ48

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	June 12, 2007
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program