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A5GIJ6

- A5GIJ6_SYNPW

UniProt

A5GIJ6 - A5GIJ6_SYNPW

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Synechococcus sp. (strain WH7803)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 1 (12 Jun 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotationSAAS annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotationSAAS annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotationSAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei584 – 5841Aminoacyl-adenylateUniRule annotation
    Binding sitei628 – 6281ATPUniRule annotation
    Metal bindingi938 – 9381ZincUniRule annotation
    Metal bindingi941 – 9411ZincUniRule annotation
    Metal bindingi958 – 9581ZincUniRule annotation
    Metal bindingi961 – 9611ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotationImported, Ligase

    Keywords - Biological processi

    Protein biosynthesisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, ZincUniRule annotationSAAS annotation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Gene namesi
    Name:ileSUniRule annotationImported
    Ordered Locus Names:SynWH7803_0335Imported
    OrganismiSynechococcus sp. (strain WH7803)Imported
    Taxonomic identifieri32051 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
    ProteomesiUP000001566: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotationSAAS annotation

    Protein-protein interaction databases

    STRINGi32051.SynWH7803_0335.

    Structurei

    3D structure databases

    ProteinModelPortaliA5GIJ6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi68 – 7811"HIGH" regionUniRule annotationAdd
    BLAST
    Motifi625 – 6295"KMSKS" regionUniRule annotation

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiEVMDEIT.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A5GIJ6-1 [UniParc]FASTAAdd to Basket

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    MSKETRDAAE GRPSYKDTLN LLQTGFGMRA NAVKREPELQ AFWRDNGIDG    50
    QLGLNNSGPT FTLHDGPPYA NGALHMGHAL NKVLKDVINK YQVLQGRRVR 100
    YVPGWDCHGL PIELKVLQSM DQEQRQALTP IKLRKKAAAY ARKQVDGQMK 150
    GFQRWGIWAD WDQPYLTLQK EYEAAQIKVF GEMVLKGHIY RGLKPVHWSP 200
    SSRTALAEAE LEYPDGHTSP SVYVAFPAVE VPVPLRETLQ SHGVELPADA 250
    AALGQALQVA IWTTTPWTLP ANLAVSVNER LDYALVDDGN GRLLVVAAEL 300
    IESLSKTLER PLQQRATVKG ALLAGLVYRH PLLDRTSPVV IGGDYITTES 350
    GTGLVHTAPG HGVDDFHTGQ KHGLPVLCPV DEAGTLTAEA GPFAGLNVLK 400
    DANPGIIEAL EQAGALLKQE AYSHRYPYDW RTKKPTIFRA TEQWFASVEG 450
    FRQDALDAID QVQWTPASGR NRIEAMVKER GDWCISRQRT WGVPIPVFYH 500
    RSNGEVLLNA DTLSHIETLI AAHGGDVWWE KDEADLLPPA YADQADQWRK 550
    GTDTMDVWFD SGSSWAAVSS QRESLSYPAD LYLEGSDQHR GWFQSSLLTS 600
    VAVNGHAPYK RVLTHGFALD EKGRKMSKSL GNVVDPMVII EGGKNQKQEP 650
    AYGADVLRLW VSSVDYSTDV PIGAGILRQL ADVYRKVRNT SRYLLGNLHD 700
    FNPATDAIAV EDLPLLDRWM LQRTAEVMDE ITEAFESYEF FRFFQLLQNF 750
    CVTDLSNFYL DIAKDRLYVS APADRRRRSC QTVMALIIER LAGLIAPVLC 800
    HMAEDIWQNL PYPVEETSVF QRGWPTAPDR WRDASLNEPM QQLRELRTSV 850
    NKVLEDCRSR GELGASLEAA VRLEAHNPSL QSALQWLNDQ GHAEVDGLRD 900
    WLLVSQLQIG GEPWAELLAN HDDDVALIEV ARARGSKCER CWHYESDVGQ 950
    HADHPHLCGR CVSVLGRL 968
    Length:968
    Mass (Da):108,194
    Last modified:June 12, 2007 - v1
    Checksum:i022836DCB2D0BA68
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CT971583 Genomic DNA. Translation: CAK22761.1.
    RefSeqiWP_011932261.1. NC_009481.1.
    YP_001224058.1. NC_009481.1.

    Genome annotation databases

    EnsemblBacteriaiCAK22761; CAK22761; SynWH7803_0335.
    GeneIDi5146786.
    KEGGisyx:SynWH7803_0335.
    PATRICi23826716. VBISynSp43824_0339.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CT971583 Genomic DNA. Translation: CAK22761.1 .
    RefSeqi WP_011932261.1. NC_009481.1.
    YP_001224058.1. NC_009481.1.

    3D structure databases

    ProteinModelPortali A5GIJ6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 32051.SynWH7803_0335.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAK22761 ; CAK22761 ; SynWH7803_0335 .
    GeneIDi 5146786.
    KEGGi syx:SynWH7803_0335.
    PATRICi 23826716. VBISynSp43824_0339.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi EVMDEIT.
    OrthoDBi EOG644ZM1.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Genoscope
      Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: WH7803Imported.

    Entry informationi

    Entry nameiA5GIJ6_SYNPW
    AccessioniPrimary (citable) accession number: A5GIJ6
    Entry historyi
    Integrated into UniProtKB/TrEMBL: June 12, 2007
    Last sequence update: June 12, 2007
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3