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A5GIJ6

- A5GIJ6_SYNPW

UniProt

A5GIJ6 - A5GIJ6_SYNPW

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Protein
Isoleucine--tRNA ligase
Gene
ileS, SynWH7803_0335
Organism
Synechococcus sp. (strain WH7803)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotationSAAS annotations

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotationSAAS annotations

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotationSAAS annotations

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei584 – 5841Aminoacyl-adenylate By similarityUniRule annotation
Binding sitei628 – 6281ATP By similarityUniRule annotation
Metal bindingi938 – 9381Zinc By similarityUniRule annotation
Metal bindingi941 – 9411Zinc By similarityUniRule annotation
Metal bindingi958 – 9581Zinc By similarityUniRule annotation
Metal bindingi961 – 9611Zinc By similarityUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotationsImported, Ligase

Keywords - Biological processi

Protein biosynthesisUniRule annotationSAAS annotations

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotations, Metal-bindingUniRule annotationSAAS annotations, Nucleotide-binding, ZincUniRule annotationSAAS annotations

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetase
Gene namesi
Name:ileSUniRule annotationImported
Ordered Locus Names:SynWH7803_0335Imported
OrganismiSynechococcus sp. (strain WH7803)
Taxonomic identifieri32051 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
ProteomesiUP000001566: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotationSAAS annotations

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotations

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotationSAAS annotations

Protein-protein interaction databases

STRINGi32051.SynWH7803_0335.

Structurei

3D structure databases

ProteinModelPortaliA5GIJ6.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi68 – 7811"HIGH" region By similarityUniRule annotation
Add
BLAST
Motifi625 – 6295"KMSKS" region By similarityUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiEVMDEIT.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5GIJ6-1 [UniParc]FASTAAdd to Basket

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MSKETRDAAE GRPSYKDTLN LLQTGFGMRA NAVKREPELQ AFWRDNGIDG    50
QLGLNNSGPT FTLHDGPPYA NGALHMGHAL NKVLKDVINK YQVLQGRRVR 100
YVPGWDCHGL PIELKVLQSM DQEQRQALTP IKLRKKAAAY ARKQVDGQMK 150
GFQRWGIWAD WDQPYLTLQK EYEAAQIKVF GEMVLKGHIY RGLKPVHWSP 200
SSRTALAEAE LEYPDGHTSP SVYVAFPAVE VPVPLRETLQ SHGVELPADA 250
AALGQALQVA IWTTTPWTLP ANLAVSVNER LDYALVDDGN GRLLVVAAEL 300
IESLSKTLER PLQQRATVKG ALLAGLVYRH PLLDRTSPVV IGGDYITTES 350
GTGLVHTAPG HGVDDFHTGQ KHGLPVLCPV DEAGTLTAEA GPFAGLNVLK 400
DANPGIIEAL EQAGALLKQE AYSHRYPYDW RTKKPTIFRA TEQWFASVEG 450
FRQDALDAID QVQWTPASGR NRIEAMVKER GDWCISRQRT WGVPIPVFYH 500
RSNGEVLLNA DTLSHIETLI AAHGGDVWWE KDEADLLPPA YADQADQWRK 550
GTDTMDVWFD SGSSWAAVSS QRESLSYPAD LYLEGSDQHR GWFQSSLLTS 600
VAVNGHAPYK RVLTHGFALD EKGRKMSKSL GNVVDPMVII EGGKNQKQEP 650
AYGADVLRLW VSSVDYSTDV PIGAGILRQL ADVYRKVRNT SRYLLGNLHD 700
FNPATDAIAV EDLPLLDRWM LQRTAEVMDE ITEAFESYEF FRFFQLLQNF 750
CVTDLSNFYL DIAKDRLYVS APADRRRRSC QTVMALIIER LAGLIAPVLC 800
HMAEDIWQNL PYPVEETSVF QRGWPTAPDR WRDASLNEPM QQLRELRTSV 850
NKVLEDCRSR GELGASLEAA VRLEAHNPSL QSALQWLNDQ GHAEVDGLRD 900
WLLVSQLQIG GEPWAELLAN HDDDVALIEV ARARGSKCER CWHYESDVGQ 950
HADHPHLCGR CVSVLGRL 968
Length:968
Mass (Da):108,194
Last modified:June 12, 2007 - v1
Checksum:i022836DCB2D0BA68
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CT971583 Genomic DNA. Translation: CAK22761.1.
RefSeqiWP_011932261.1. NC_009481.1.
YP_001224058.1. NC_009481.1.

Genome annotation databases

EnsemblBacteriaiCAK22761; CAK22761; SynWH7803_0335.
GeneIDi5146786.
KEGGisyx:SynWH7803_0335.
PATRICi23826716. VBISynSp43824_0339.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CT971583 Genomic DNA. Translation: CAK22761.1 .
RefSeqi WP_011932261.1. NC_009481.1.
YP_001224058.1. NC_009481.1.

3D structure databases

ProteinModelPortali A5GIJ6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 32051.SynWH7803_0335.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAK22761 ; CAK22761 ; SynWH7803_0335 .
GeneIDi 5146786.
KEGGi syx:SynWH7803_0335.
PATRICi 23826716. VBISynSp43824_0339.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi EVMDEIT.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Genoscope
    Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: WH7803.

Entry informationi

Entry nameiA5GIJ6_SYNPW
AccessioniPrimary (citable) accession number: A5GIJ6
Entry historyi
Integrated into UniProtKB/TrEMBL: June 12, 2007
Last sequence update: June 12, 2007
Last modified: September 3, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

Similar proteinsi