Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Synechococcus sp. (strain WH7803)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation
Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) (By similarity).SAAS annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotationSAAS annotation

Cofactori

Zn2+UniRule annotationSAAS annotationNote: Binds 1 zinc ion per subunit.UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei584 – 5841Aminoacyl-adenylateUniRule annotation
Binding sitei628 – 6281ATPUniRule annotation
Metal bindingi938 – 9381ZincUniRule annotation
Metal bindingi941 – 9411ZincUniRule annotation
Metal bindingi958 – 9581ZincUniRule annotation
Metal bindingi961 – 9611ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotation, Ligase

Keywords - Biological processi

Protein biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, ZincUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotationSAAS annotation (EC:6.1.1.5UniRule annotationSAAS annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Gene namesi
Name:ileSUniRule annotationImported
Ordered Locus Names:SynWH7803_0335Imported
OrganismiSynechococcus sp. (strain WH7803)Imported
Taxonomic identifieri32051 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
ProteomesiUP000001566 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Monomer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi32051.SynWH7803_0335.

Structurei

3D structure databases

ProteinModelPortaliA5GIJ6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi68 – 7811"HIGH" regionUniRule annotationAdd
BLAST
Motifi625 – 6295"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5GIJ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKETRDAAE GRPSYKDTLN LLQTGFGMRA NAVKREPELQ AFWRDNGIDG
60 70 80 90 100
QLGLNNSGPT FTLHDGPPYA NGALHMGHAL NKVLKDVINK YQVLQGRRVR
110 120 130 140 150
YVPGWDCHGL PIELKVLQSM DQEQRQALTP IKLRKKAAAY ARKQVDGQMK
160 170 180 190 200
GFQRWGIWAD WDQPYLTLQK EYEAAQIKVF GEMVLKGHIY RGLKPVHWSP
210 220 230 240 250
SSRTALAEAE LEYPDGHTSP SVYVAFPAVE VPVPLRETLQ SHGVELPADA
260 270 280 290 300
AALGQALQVA IWTTTPWTLP ANLAVSVNER LDYALVDDGN GRLLVVAAEL
310 320 330 340 350
IESLSKTLER PLQQRATVKG ALLAGLVYRH PLLDRTSPVV IGGDYITTES
360 370 380 390 400
GTGLVHTAPG HGVDDFHTGQ KHGLPVLCPV DEAGTLTAEA GPFAGLNVLK
410 420 430 440 450
DANPGIIEAL EQAGALLKQE AYSHRYPYDW RTKKPTIFRA TEQWFASVEG
460 470 480 490 500
FRQDALDAID QVQWTPASGR NRIEAMVKER GDWCISRQRT WGVPIPVFYH
510 520 530 540 550
RSNGEVLLNA DTLSHIETLI AAHGGDVWWE KDEADLLPPA YADQADQWRK
560 570 580 590 600
GTDTMDVWFD SGSSWAAVSS QRESLSYPAD LYLEGSDQHR GWFQSSLLTS
610 620 630 640 650
VAVNGHAPYK RVLTHGFALD EKGRKMSKSL GNVVDPMVII EGGKNQKQEP
660 670 680 690 700
AYGADVLRLW VSSVDYSTDV PIGAGILRQL ADVYRKVRNT SRYLLGNLHD
710 720 730 740 750
FNPATDAIAV EDLPLLDRWM LQRTAEVMDE ITEAFESYEF FRFFQLLQNF
760 770 780 790 800
CVTDLSNFYL DIAKDRLYVS APADRRRRSC QTVMALIIER LAGLIAPVLC
810 820 830 840 850
HMAEDIWQNL PYPVEETSVF QRGWPTAPDR WRDASLNEPM QQLRELRTSV
860 870 880 890 900
NKVLEDCRSR GELGASLEAA VRLEAHNPSL QSALQWLNDQ GHAEVDGLRD
910 920 930 940 950
WLLVSQLQIG GEPWAELLAN HDDDVALIEV ARARGSKCER CWHYESDVGQ
960
HADHPHLCGR CVSVLGRL
Length:968
Mass (Da):108,194
Last modified:June 12, 2007 - v1
Checksum:i022836DCB2D0BA68
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CT971583 Genomic DNA. Translation: CAK22761.1.
RefSeqiWP_011932261.1. NC_009481.1.

Genome annotation databases

EnsemblBacteriaiCAK22761; CAK22761; SynWH7803_0335.
KEGGisyx:SynWH7803_0335.
PATRICi23826716. VBISynSp43824_0339.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CT971583 Genomic DNA. Translation: CAK22761.1.
RefSeqiWP_011932261.1. NC_009481.1.

3D structure databases

ProteinModelPortaliA5GIJ6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi32051.SynWH7803_0335.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAK22761; CAK22761; SynWH7803_0335.
KEGGisyx:SynWH7803_0335.
PATRICi23826716. VBISynSp43824_0339.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Genoscope
    Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: WH7803Imported.

Entry informationi

Entry nameiA5GIJ6_SYNPW
AccessioniPrimary (citable) accession number: A5GIJ6
Entry historyi
Integrated into UniProtKB/TrEMBL: June 12, 2007
Last sequence update: June 12, 2007
Last modified: July 22, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.