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Protein

tRNA dimethylallyltransferase

Gene

miaA

Organism
Synechococcus sp. (strain WH7803)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i6A).UniRule annotation

Catalytic activityi

Dimethylallyl diphosphate + adenine(37) in tRNA = diphosphate + N(6)-dimethylallyladenine(37) in tRNA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi24 – 31ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA dimethylallyltransferaseUniRule annotation (EC:2.5.1.75UniRule annotation)
Alternative name(s):
Dimethylallyl diphosphate:tRNA dimethylallyltransferaseUniRule annotation
Short name:
DMAPP:tRNA dimethylallyltransferaseUniRule annotation
Short name:
DMATaseUniRule annotation
Isopentenyl-diphosphate:tRNA isopentenyltransferaseUniRule annotation
Short name:
IPP transferaseUniRule annotation
Short name:
IPPTUniRule annotation
Short name:
IPTaseUniRule annotation
Gene namesi
Name:miaAUniRule annotation
Ordered Locus Names:SynWH7803_0146
OrganismiSynechococcus sp. (strain WH7803)
Taxonomic identifieri32051 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesSynechococcaceaeSynechococcus
Proteomesi
  • UP000001566 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003773491 – 310tRNA dimethylallyltransferaseAdd BLAST310

Interactioni

Subunit structurei

Monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei115Interaction with substrate tRNAUniRule annotation1

Protein-protein interaction databases

STRINGi32051.SynWH7803_0146.

Structurei

3D structure databases

ProteinModelPortaliA5GI07.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni26 – 31Substrate bindingUniRule annotation6
Regioni49 – 52Interaction with substrate tRNAUniRule annotation4

Sequence similaritiesi

Belongs to the IPP transferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DKX. Bacteria.
COG0324. LUCA.
HOGENOMiHOG000039996.
KOiK00791.
OMAiARKQLTW.
OrthoDBiPOG091H00NK.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans. 1 hit.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00174. miaA. 1 hit.

Sequencei

Sequence statusi: Complete.

A5GI07-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPHTSPETEA QRDSNAPLVV VLLGPTASGK TALALELAER FDLEIINVDS
60 70 80 90 100
RQLYQEMSVG TAKPSPEQQA RIRHHLLDLR PPDQPITLQE FQEEALQAVN
110 120 130 140 150
QSLAKRGAAF LVGGSGLYLK ALTAGLRPPA VAPQPALRRQ LAQLGQPLCH
160 170 180 190 200
QLLNTADPEA AVRIASADAL RTQRALEVLY ATGAPMSRQT SASPPPWRVL
210 220 230 240 250
ELGLNPLDLR QRISARTQAL YSQGLVEETR QLRERYGPEL PLLQTIGYGE
260 270 280 290 300
ALQVLAGDLS RPAAIAHTTR RTQQFAKRQR TWFRRQHQPH WLPDDNPLNE
310
AGRLIEAGLG
Length:310
Mass (Da):34,173
Last modified:June 12, 2007 - v1
Checksum:iA86632E0D35B1177
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CT971583 Genomic DNA. Translation: CAK22572.1.
RefSeqiWP_011932080.1. NC_009481.1.

Genome annotation databases

EnsemblBacteriaiCAK22572; CAK22572; SynWH7803_0146.
KEGGisyx:SynWH7803_0146.
PATRICi23826326. VBISynSp43824_0147.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CT971583 Genomic DNA. Translation: CAK22572.1.
RefSeqiWP_011932080.1. NC_009481.1.

3D structure databases

ProteinModelPortaliA5GI07.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi32051.SynWH7803_0146.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAK22572; CAK22572; SynWH7803_0146.
KEGGisyx:SynWH7803_0146.
PATRICi23826326. VBISynSp43824_0147.

Phylogenomic databases

eggNOGiENOG4105DKX. Bacteria.
COG0324. LUCA.
HOGENOMiHOG000039996.
KOiK00791.
OMAiARKQLTW.
OrthoDBiPOG091H00NK.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans. 1 hit.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00174. miaA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMIAA_SYNPW
AccessioniPrimary (citable) accession number: A5GI07
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: June 12, 2007
Last modified: November 2, 2016
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.