ID   PYRC_GEOUR              Reviewed;         425 AA.
AC   A5GF42;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-JUN-2007, sequence version 1.
DT   16-JUN-2009, entry version 16.
DE   RecName: Full=Dihydroorotase;
DE            Short=DHOase;
DE            EC=3.5.2.3;
GN   Name=pyrC; OrderedLocusNames=Gura_1857;
OS   Geobacter uraniireducens (strain Rf4) (Geobacter uraniumreducens).
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Desulfuromonadales;
OC   Geobacteraceae; Geobacter.
OX   NCBI_TaxID=351605;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H.,
RA   Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N.,
RA   Shelobolina E., Aklujkar M., Lovley D., Richardson P.;
RT   "Complete sequence of Geobacter uraniireducens Rf4.";
RL   Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: (S)-dihydroorotate + H(2)O = N-carbamoyl-L-
CC       aspartate.
CC   -!- COFACTOR: Binds 2 zinc ions per subunit (By similarity).
CC   -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo
CC       pathway; UMP from HCO(3)(-): step 3/6.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SIMILARITY: Belongs to the DHOase family. Type 2 subfamily.
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DR   EMBL; CP000698; ABQ26047.1; -; Genomic_DNA.
DR   RefSeq; YP_001230620.1; -.
DR   GeneID; 5166201; -.
DR   GenomeReviews; CP000698_GR; Gura_1857.
DR   KEGG; gur:Gura_1857; -.
DR   NMPDR; fig|351605.3.peg.377; -.
DR   OMA; A5GF42; CDVHPVG.
DR   GO; GO:0004151; F:dihydroorotase activity; IEA:HAMAP.
DR   GO; GO:0008270; F:zinc ion binding; IEA:HAMAP.
DR   GO; GO:0006221; P:pyrimidine nucleotide biosynthetic process; IEA:HAMAP.
DR   HAMAP; MF_00220; -; 1.
DR   InterPro; IPR006680; Amidohydro_1.
DR   InterPro; IPR004722; DHOmult.
DR   InterPro; IPR002195; Dihydroorotase_CS.
DR   Pfam; PF01979; Amidohydro_1; 1.
DR   TIGRFAMs; TIGR00857; pyrC_multi; 1.
DR   PROSITE; PS00482; DIHYDROOROTASE_1; 1.
DR   PROSITE; PS00483; DIHYDROOROTASE_2; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Hydrolase; Metal-binding; Pyrimidine biosynthesis;
KW   Zinc.
FT   CHAIN         1    425       Dihydroorotase.
FT                                /FTId=PRO_1000078107.
FT   METAL        61     61       Zinc 1 (By similarity).
FT   METAL        63     63       Zinc 1 (By similarity).
FT   METAL       143    143       Zinc 1; via carbamate group (By
FT                                similarity).
FT   METAL       143    143       Zinc 2; via carbamate group (By
FT                                similarity).
FT   METAL       180    180       Zinc 2 (By similarity).
FT   METAL       233    233       Zinc 2 (By similarity).
FT   METAL       306    306       Zinc 1 (By similarity).
FT   MOD_RES     143    143       N6-carboxylysine (By similarity).
SQ   SEQUENCE   425 AA;  44938 MW;  AC06964FA760C291 CRC64;
     MNLLIKGGRV VDPSQNIDDT MDLLVEDGRI KEIGKGLKAP AGAEIIDAAG LLVTPGLIDM
     HVHLRDPGLE YKEDIVTGTR AAAAGGFTSV ACMPNTKPVN DNKAITSYIV NKAAKEALVN
     VFPVGSITQG SKGESLAEMG ELKESGCVAV SDDGRPVVNG ELMRRALEYA KGMGIMVISH
     SEELALVGEG VMNEGFTATE LGLKGIPWAA EDVAVARDVY LAEFTDSPLH IAHISTSGSV
     RIIRNAKARG VKVTCETAPH YFSLTDDAVR GYNTNAKMNP PLREAADVAA IKAGLADGTI
     DAIATDHAPH HLDEKDLEFN LALNGIVGLE TSLPLSLQLV EEGVVDLKVL LEKMTCNPAK
     ILGIDRGTLK VGAAADITVI DPDREWLVAA EKLASKSKNS PFIGRKMKGA AVYTVVGGKV
     VYKID
//