Reviewed, UniProtKB/Swiss-Prot A5GCZ4 (NUBCD_GEOUR)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit B/C/D
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit B/C/D
    NDH-1 subunit B/C/D

Gene names

Name:	nuoBCD
Synonyms:	nuoB, nuoC, nuoD
Ordered Locus Names:	Gura_0333

Organism

Geobacter uraniireducens (strain Rf4) (Geobacter uraniumreducens) [Complete proteome] [HAMAP]

Taxonomic identifier

351605 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfuromonadales › Geobacteraceae › Geobacter

Protein attributes

Sequence length	793 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01356
Subunit structure	NDH-1 is composed of about 13 different subunits. Subunits nuoBCD, E, F, and G constitute the peripheral sector of the complex By similarity.
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity.
Sequence similarities	In the N-terminal section; belongs to the complex I 20 kDa subunit family. In the central section; belongs to the complex I 30 kDa subunit family. In the C-terminal section; belongs to the complex I 49 kDa subunit family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: HAMAP photosynthesis, light reaction Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	internal side of plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: HAMAP quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 793	793	NADH-quinone oxidoreductase subunit B/C/D HAMAP MF_01356		PRO_0000358645
Regions
	Region	1 – 157	157	NADH dehydrogenase I subunit B By similarity
	Region	231 – 386	156	NADH dehydrogenase I subunit C By similarity
	Region	413 – 793	381	NADH dehydrogenase I subunit D By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5GCZ4-1 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: A4DA445AE43EA6A3	FASTA	793	89,868
	10 20 30 40 50 60 MTEQDATPHN NVILANLDDL INWGRANSLW PMFFGLSCCF VEMMTSFTSR YDISRFGAEV 70 80 90 100 110 120 LRGTPREADL MVIAGTVFKK MAPSILRLYE QMAEPRWVIS MGSCANSGGM YDVYSVVQGV 130 140 150 160 170 180 NQIIPVDVYV PGCPPRPEAF LQGLMLLQEK IKTCERPARP VLHMQGGSQG TTTPVLVDGV 190 200 210 220 230 240 SKSRDTRGPG MEATSIRGTS VQHPKFLLPR SDEMWRPPAA KHAYPDFDLT GELELAFDHR 250 260 270 280 290 300 LTVDRNATDM LTYRCPAELV PDVLKHLKQR GSKPFRRLED IAAVDESCRR ERGKFRDFTV 310 320 330 340 350 360 NYHLLCFDTP GHIRIKTELS GASPELPTAT AVFSAANWYE REVFDMYGIR FSGHPDLRRI 370 380 390 400 410 420 LMPPDWEGHP LRKEHPFRAT EMPPYTTDDA RKLQPLPAGD FFDRLDEETL ILNLGPQHPG 430 440 450 460 470 480 THGIIRFILK LKGEEIVDMD TDIGFHHRGA EKMGERQHWN QFIPYTDRID YLAGVQNNLA 490 500 510 520 530 540 YVNSVERLCS ITVPDRAVYI RVMLAELFRI ASHLVWLGTF AADVGAMTPV FYTFTDREKI 550 560 570 580 590 600 FDIVEMITGG RMHPAWFRIG GVAEDLPEGW EEPVKAFLAW FPPRLKEYEE LLNGNPIFKA 610 620 630 640 650 660 RLKGVGAIGQ AEAIDWGVSG PNLRACGLEW DLRKKMPYAG YERFHFDVAV AEGGDCYARY 670 680 690 700 710 720 LVRIEEMKQS LHIVRQCLHD MPGGRWITDD YRFVLPQKRD TLNDIESLIH HFVNSTRGMA 730 740 750 760 770 780 PPPGECYTAI EAPKGENGYF VVSDGINIPY RVRIKTPSFP HIQALPLMSK GWLVADFLAI 790 IGSIDFVLAD LDR

References

[1]

"Complete sequence of Geobacter uraniireducens Rf4." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Shelobolina E., Aklujkar M., Lovley D., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000698 Genomic DNA. Translation: ABQ24549.1.
RefSeq	YP_001229122.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A5GCZ4.
Genome annotation databases
GeneID	5164573.
GenomeReviews	Gene locus Gura_0333 in contig CP000698_GR.
KEGG	gur:Gura_0333.
NMPDR	fig|351605.3.peg.1041.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	PPTWEGH.
Family and domain databases
HAMAP	MF_01356. Fused. [Tree] MF_01359. Fused. [Tree]
InterPro	IPR010219. NADH_DH_1_dsu. IPR010218. NADH_DH_csu. IPR001135. NADH_Q_OxRdtase_suD. IPR006137. NADH_UbQ_OxRdtase-like_20kDa. IPR006138. NADH_UbQ_OxRdtase_20kDa_su. IPR001268. NADH_UbQ_OxRdtase_30kDa_su. IPR014029. NADH_UbQ_OxRdtase_49kDa_CS. IPR020396. NADH_UbQ_OxRdtase_CS. [Graphical view]
Pfam	PF00329. Complex1_30kDa. 1 hit. PF00346. Complex1_49kDa. 1 hit. PF01058. Oxidored_q6. 1 hit. [Graphical view]
ProDom	PD001581. Complex1_30K. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01957. nuoB_fam. 1 hit. TIGR01961. NuoC_fam. 1 hit. TIGR01962. NuoD. 1 hit.
PROSITE	PS01150. COMPLEX1_20K. False negative. PS00542. COMPLEX1_30K. 1 hit. PS00535. COMPLEX1_49K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUBCD_GEOUR

Accession

Primary (citable) accession number: A5GCZ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	June 12, 2007
Last modified:	November 3, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)