Reviewed, UniProtKB/Swiss-Prot A5G9B9 (NUOA2_GEOUR)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit A 2
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit A 2
    NDH-1 subunit A 2
    NUO1 2

Gene names

Name:	nuoA2
Ordered Locus Names:	Gura_4244

Organism

Geobacter uraniireducens (strain Rf4) (Geobacter uraniumreducens) [Complete proteome] [HAMAP]

Taxonomic identifier

351605 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfuromonadales › Geobacteraceae › Geobacter

Protein attributes

Sequence length	118 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01394
Subunit structure	NDH-1 is composed of 14 different subunits. Subunits nuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the complex I subunit 3 family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: HAMAP photosynthesis, light reaction Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 118	118	NADH-quinone oxidoreductase subunit A 2 HAMAP MF_01394		PRO_0000362696
Regions
	Transmembrane	5 – 25	21	Potential
	Transmembrane	62 – 82	21	Potential
	Transmembrane	87 – 107	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	A5G9B9-1 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: C641AA4A132251C9	FASTA	118	13,318
	10 20 30 40 50 60 MLGAYLPIIV LVVVAVLFGC GSLIFSSLIG QKKPSVVKMA PYECGCEPVG SARERFSIKF 70 80 90 100 110 YIIAMLFILF DIEAVFLYPW AVLFKRLGMF GLMEMGVFIV ILFVGYIYVW KKGALEWE

References

[1]

"Complete sequence of Geobacter uraniireducens Rf4." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Shelobolina E., Aklujkar M., Lovley D., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000698 Genomic DNA. Translation: ABQ28387.1.
RefSeq	YP_001232960.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A5G9B9.
Genome annotation databases
GeneID	5165425.
GenomeReviews	Gene locus Gura_4244 in contig CP000698_GR.
KEGG	gur:Gura_4244.
NMPDR	fig|351605.3.peg.2428.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	PKRYNRA.
Family and domain databases
HAMAP	MF_01394. [Tree]
InterPro	IPR000440. NADH_UbQ/plastoQ_OxRdtase_su3. [Graphical view]
PANTHER	PTHR11058. Oxidored_q4. 1 hit.
Pfam	PF00507. Oxidored_q4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOA2_GEOUR

Accession

Primary (citable) accession number: A5G9B9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	June 12, 2007
Last modified:	November 3, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)