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A5G8J7 (DDL_GEOUR) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Gura_3971
OrganismGeobacter uraniireducens (strain Rf4) (Geobacter uraniumreducens) [Complete proteome] [HAMAP]
Taxonomic identifier351605 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter

Protein attributes

Sequence length310 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 310310D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341103

Regions

Domain107 – 302196ATP-grasp
Nucleotide binding135 – 18854ATP By similarity

Sites

Metal binding2561Magnesium or manganese 1 By similarity
Metal binding2691Magnesium or manganese 1 By similarity
Metal binding2691Magnesium or manganese 2 By similarity
Metal binding2711Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A5G8J7 [UniParc].

Last modified June 12, 2007. Version 1.
Checksum: 2CDAFEF14D889EFC

FASTA31033,482
        10         20         30         40         50         60 
MTVDELKTKK IGVLMGGLSA EREVSLKSGA AVHQALLARG YDAVAIDVDR DIAQVLVREW 

        70         80         90        100        110        120 
VDVAFIALHG RYGEDGAIQG LLEIMGIPYT GSGVLASALA MNKIFAKQAF QAARLTVAPY 

       130        140        150        160        170        180 
KVFCRGDKAA LAELEFSLPV VVKPSQEGSS VGVSIVKKES EFAAAMKEAF RYDREILVEQ 

       190        200        210        220        230        240 
FIKGSEVQVG ILEDKALGAI EIVPKNEFYD FDAKYSPGMA EHILPARLPA ELYRKVLRAG 

       250        260        270        280        290        300 
EDAHRALGCS GYSRVDFLVT EEGACYILEV NTLPGMTDLS LLPEIARGSG IGFEDLVERI 

       310 
LAAAALKISQ 

« Hide

References

[1]"Complete sequence of Geobacter uraniireducens Rf4."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Mikhailova N., Shelobolina E., Aklujkar M., Lovley D., Richardson P.
Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Rf4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000698 Genomic DNA. Translation: ABQ28115.1.
RefSeqYP_001232688.1. NC_009483.1.

3D structure databases

ProteinModelPortalA5G8J7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING351605.Gura_3971.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ28115; ABQ28115; Gura_3971.
GeneID5162799.
KEGGgur:Gura_3971.
PATRIC22038377. VBIGeoUra13052_4236.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMACSGYSRV.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycGURA351605:GI6A-4024-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_GEOUR
AccessionPrimary (citable) accession number: A5G8J7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 12, 2007
Last modified: May 14, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways