A5G7Y9 (NADE_GEOUR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	Gura_3754

Organism

Geobacter uraniireducens (strain Rf4) (Geobacter uraniumreducens) [Complete proteome] [HAMAP]

Taxonomic identifier

351605 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfuromonadales › Geobacteraceae › Geobacter

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000077558
Regions
	Nucleotide binding	34 – 41	8	ATP By similarity
Sites
	Active site	36	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5G7Y9 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: E1415FC92F8DF461	FASTA	273	30,787
	10 20 30 40 50 60 MAGLAMNTEL LRRILVGFIR EEVRKIGLQK AVLGLSGGID SALVAYLAAE ALGPENVYAC 70 80 90 100 110 120 TMPYRTSNPE SEAHARLVAE RLGINYRVIE ITPMVDAYFQ MVPDADNMRR GNKMARERMT 130 140 150 160 170 180 ILYDHSAAYS ALVLGTSNKT ELLLGYGTLF GDMASALNPI GDIYKTQVWQ LSEAMGVPRE 190 200 210 220 230 240 VIEKKPSADL WAGQTDEQEL GFTYREVDEL LYQMVDLRCN RQELIAQGFS ADFIDKIYGK 250 260 270 VQNSHFKRRL PVIAKVSTRT IDRDFRYSRD WGK

References

[1]

"Complete sequence of Geobacter uraniireducens Rf4." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Shelobolina E., Aklujkar M., Lovley D., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Rf4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000698 Genomic DNA. Translation: ABQ27907.1.
RefSeq	YP_001232480.1. NC_009483.1.
3D structure databases
ProteinModelPortal	A5G7Y9.
ModBase	Search...
Protein-protein interaction databases
STRING	A5G7Y9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5166664.
GenomeReviews	Gene locus Gura_3754 in contig CP000698_GR.
KEGG	gur:Gura_3754.
NMPDR	fig|351605.3.peg.1922.
PATRIC	22037920. VBIGeoUra13052_4009.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0171.
HOGENOM	HBG351567.
OMA	DGAVDCH.
ProtClustDB	PRK13980.
Enzyme and pathway databases
BioCyc	GURA351605:GURA_3754-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_GEOUR

Accession

Primary (citable) accession number: A5G7Y9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	June 12, 2007
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program