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A5G677 (SYL_GEOUR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Gura_3134
OrganismGeobacter uraniireducens (strain Rf4) (Geobacter uraniumreducens) [Complete proteome] [HAMAP]
Taxonomic identifier351605 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter

Protein attributes

Sequence length824 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 824824Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000074835

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif581 – 5855"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5841ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A5G677 [UniParc].

Last modified June 12, 2007. Version 1.
Checksum: 5AA28FA742CB2ABA

FASTA82493,456
        10         20         30         40         50         60 
MEEKYLPSRV EEKWQKFWEA NKSFRATEDK TREKYYLLEM FPYPSGRIHM GHVRNYSIGD 

        70         80         90        100        110        120 
VIARFKRMKG FNVLHPMGWD AFGMPAENAA IQNKSHPAKW TYENIAYMRG QLKKMGLSYD 

       130        140        150        160        170        180 
WDRELATCDV DYYKWEQLIF LQMYKKGLAY KKISSVNWCP KCETVLANEQ VEDGCCWRCD 

       190        200        210        220        230        240 
SNVDQKELEQ WSFRITDYAE ELLEYTYKLP GWPERVLTMQ RNWIGRSTGC EIDFPIEGQM 

       250        260        270        280        290        300 
DKIKVFTTRQ DTLFGATFMS LAPEHPMALE LTTPENMATV AAFIEKVKKT DRIRRTAEDF 

       310        320        330        340        350        360 
EKEGVFTGSY CINPVTNRRM PVYLANFVLT DYGTGAVMAV PTHDQRDFEF ARKYDIPMGV 

       370        380        390        400        410        420 
VIQPEGEALD PQTMAEAFTA EGIMVNSGRF DGLKSGAAKE QIADYLEKEG IGKKTVNYRL 

       430        440        450        460        470        480 
RDWGISRQRY WGNPIPMIYC DLCGAVPVPE TDLPVVLPMD ATFTGEGGNP LDKVDSFVNT 

       490        500        510        520        530        540 
TCPQCGEAAR RETDTMDTFV ESSWYFLRYC CPDFVSGPLD KEKTEYWMSV DQYIGGIEHA 

       550        560        570        580        590        600 
VMHLLYARFF TKVLRDLGYC DIDEPFTNLL TQGMVIKDGA KMSKSKGNVV DPNALIDKYG 

       610        620        630        640        650        660 
ADTARLFSLF AAPPEKDLDW SDQGVDGSFR FLNRVWKLVY DTLPIIGGAG ALDPAGLTNE 

       670        680        690        700        710        720 
GKGLRRQVHK TIRKVTEDIE ERFHFNTAIA ATMELVNTIQ SFEPKNSPQN APVLKEAIES 

       730        740        750        760        770        780 
VVMLLAPFVP HFTEELWAQL GNNKSLEQAG WPAFDSAAAI DEELLVVVQV NGKLRGKLTV 

       790        800        810        820 
AVTASEDDVK GAALADERVK PFIEGKSVKK IVYVPGKLVN IVVG 

« Hide

References

[1]"Complete sequence of Geobacter uraniireducens Rf4."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Mikhailova N., Shelobolina E., Aklujkar M., Lovley D., Richardson P.
Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Rf4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000698 Genomic DNA. Translation: ABQ27295.1.
RefSeqYP_001231868.1. NC_009483.1.

3D structure databases

ProteinModelPortalA5G677.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING351605.Gura_3134.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ27295; ABQ27295; Gura_3134.
GeneID5164939.
KEGGgur:Gura_3134.
PATRIC22036589. VBIGeoUra13052_3352.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycGURA351605:GI6A-3178-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_GEOUR
AccessionPrimary (citable) accession number: A5G677
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: June 12, 2007
Last modified: April 16, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries