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A5G2D2

- LIPA_ACICJ

UniProt

A5G2D2 - LIPA_ACICJ

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Protein
Lipoyl synthase
Gene
lipA, Acry_2824
Organism
Acidiphilium cryptum (strain JF-5)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi61 – 611Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi66 – 661Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi72 – 721Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi87 – 871Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi91 – 911Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi94 – 941Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciACRY349163:GHET-2876-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:Acry_2824
OrganismiAcidiphilium cryptum (strain JF-5)
Taxonomic identifieri349163 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeAcidiphilium
ProteomesiUP000000245: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 320320Lipoyl synthaseUniRule annotation
PRO_1000012179Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi349163.Acry_2824.

Structurei

3D structure databases

ProteinModelPortaliA5G2D2.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiHPHIPTK.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

A5G2D2-1 [UniParc]FASTAAdd to Basket

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MRVEIDHRNS GGGKLRHPEK QHRPDNPIQR KPAWIRVKAP NHPVYHETRA    50
LMREAKLVTV CEEAACPNIG ECWSQRHATM MIMGEICTRA CAFCNVTTGQ 100
PAPLAADEPA RVAEAVARLG LQHVVITSVD RDDLDDGGAA HFAAVIGAIR 150
AAAPSTTIEI LTPDFLRKPG ALEVVVAARP DVFNHNLETV PRLYPSIRPG 200
ARYYQSLRLL DRVKQLDPSI FTKSGLMAGL GEDRGEVGQV MDDLRIADVD 250
FLTIGQYLQP TVKHAAVDRF VTPDEFADLA AMARAKGFLM VSATPLTRSS 300
YHADADFAAL REARAARHAA 320
Length:320
Mass (Da):35,050
Last modified:June 12, 2007 - v1
Checksum:i41AE52897EE64914
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000697 Genomic DNA. Translation: ABQ32014.1.
RefSeqiYP_001235933.1. NC_009484.1.

Genome annotation databases

EnsemblBacteriaiABQ32014; ABQ32014; Acry_2824.
GeneIDi5160374.
KEGGiacr:Acry_2824.
PATRICi20650778. VBIAciCry6074_3345.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000697 Genomic DNA. Translation: ABQ32014.1 .
RefSeqi YP_001235933.1. NC_009484.1.

3D structure databases

ProteinModelPortali A5G2D2.
ModBasei Search...

Protein-protein interaction databases

STRINGi 349163.Acry_2824.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABQ32014 ; ABQ32014 ; Acry_2824 .
GeneIDi 5160374.
KEGGi acr:Acry_2824.
PATRICi 20650778. VBIAciCry6074_3345.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi HPHIPTK.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci ACRY349163:GHET-2876-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JF-5.

Entry informationi

Entry nameiLIPA_ACICJ
AccessioniPrimary (citable) accession number: A5G2D2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 12, 2007
Last modified: May 14, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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