Reviewed, UniProtKB/Swiss-Prot A5G293 (PURA_ACICJ)

Names and origin

Protein names

Recommended name:
    Adenylosuccinate synthetase
    EC=6.3.4.4
Alternative name(s):
    IMP--aspartate ligase
    AdSS
    AMPSase

Gene names

Name:	purA
Ordered Locus Names:	Acry_2784

Organism

Acidiphilium cryptum (strain JF-5) [Complete proteome] [HAMAP]

Taxonomic identifier

349163 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Acidiphilium

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays an important role in the de novo pathway of purine nucleotide biosynthesis. HAMAP MF_00011
Catalytic activity	GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: HAMAP adenylosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 429	429	Adenylosuccinate synthetase HAMAP MF_00011		PRO_1000000769
Regions
	Nucleotide binding	12 – 18	7	GTP Potential
Sites
	Active site	140	1	By similarity
	Active site	147	1	By similarity
	Metal binding	13	1	Magnesium By similarity
	Metal binding	40	1	Magnesium; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5G293-1 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: EE1D5D5B8BA11653	FASTA	429	45,483
	10 20 30 40 50 60 MANVTIIGAQ WGDEGKGKVV DWLASRADVV VRFQGGHNAG HTLVVGNQTY KLSLLPSGLV 70 80 90 100 110 120 RGKLGVIGNG VVVDPVALLA EIGRVREQGL NVTPETLRIA DNAPLILPLH AALDAAREAA 130 140 150 160 170 180 RGARRIGTTG RGIGPAYEDK VARRAIRICD LAEPETLDWR LDELLLHHNT LLAGLGAPTF 190 200 210 220 230 240 GKAELMDQLL ALAPQILPFA EPVWERLAEA KKSGARILFE GAQAVMLDVD HGTYPFVTSS 250 260 270 280 290 300 NTVSGQIAAG AGVGPDAAGR VLGIAKAYST RVGSGPFPTE QDNETGRLLG ERGHEFGTVT 310 320 330 340 350 360 GRARRCGWFD AALVRQAVKV GGIQGLALTK LDVLDGMTTL QIGVGYRIGG ETLPHLPPGA 370 380 390 400 410 420 AAQAKAEPVY ETIEGWSGST RGARSYADLP AQAIKYVRRI EELVECPVTL LSTSPERDDT ILMQDPFAD

References

[1]

"Complete sequence of chromosome of Acidiphilium cryptum JF-5." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Magnuson T., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000697 Genomic DNA. Translation: ABQ31975.1.
RefSeq	YP_001235894.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A5G293.
Genome annotation databases
GeneID	5159997.
GenomeReviews	Gene locus Acry_2784 in contig CP000697_GR.
KEGG	acr:Acry_2784.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	IPVCVAY.
Family and domain databases
HAMAP	MF_00011. [Tree]
InterPro	IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view]
PANTHER	PTHR11846. Asucc_synthtase. 1 hit.
Pfam	PF00709. Adenylsucc_synt. 1 hit. [Graphical view]
ProDom	PD001188. Asucc_synthtase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00788. Adenylsucc_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00184. purA. 1 hit.
PROSITE	PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURA_ACICJ

Accession

Primary (citable) accession number: A5G293

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 12, 2007
Last modified:	November 3, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)