A5G1Y4 (SYE2_ACICJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 2
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase 2
Short name=GluRS 2

Gene names

Name:	gltX2
Ordered Locus Names:	Acry_2675

Organism

Acidiphilium cryptum (strain JF-5) [Complete proteome] [HAMAP]

Taxonomic identifier

349163 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Acidiphilium

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 444	444	Glutamate--tRNA ligase 2 HAMAP MF_00022_B		PRO_0000367596
Regions
	Motif	8 – 18	11	"HIGH" region HAMAP MF_00022_B
	Motif	241 – 245	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	244	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5G1Y4 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 23880B67DBD31A6B	FASTA	444	48,527
	10 20 30 40 50 60 MVKFRFAPSP TGHLHAGNAR LAVVNALAAR AMGGTLLLRI DDTDRERSKP EYEAGIEQDL 70 80 90 100 110 120 RWLGIGWDEM ARQSDRMDRY AAAAERLKAA GLLYPCFESE EELAAKRALR AKRHLPPVYD 130 140 150 160 170 180 RAMLSLTAEQ RAAAEAGGKR PYFRFKLSDG AVAWNDLVLG RREVALGTVS DPVLIRADGT 190 200 210 220 230 240 PLYTFTSVVD DLELGVTHVV RGEDHVTNTA VQIDLMRALE PRRAVPAFGH LPLISDVTGE 250 260 270 280 290 300 KLSKRAGSVS VRQFRRDGIE AMALVSYLAR LGSRRDPEPL TLEELAGTFD LGDFSRGAPR 310 320 330 340 350 360 FDPKQLLALN RRVLHGLPFE AVAERLPEGC GAEFWMAVRG NLDLLSEARL WWEVVSGEIE 370 380 390 400 410 420 TPSLPEAASL LRAALEALPP EPWDETTWKG WTGAVAAASG ARGKALYLPL RLALTGESHG 430 440 PELAALLPLI GRARAAARLE RAAG

References

[1]

"Complete sequence of chromosome of Acidiphilium cryptum JF-5." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Magnuson T., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JF-5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000697 Genomic DNA. Translation: ABQ31866.1.
RefSeq	YP_001235785.1. NC_009484.1.
3D structure databases
ProteinModelPortal	A5G1Y4.
ModBase	Search...
Protein-protein interaction databases
STRING	A5G1Y4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5162132.
GenomeReviews	Gene locus Acry_2675 in contig CP000697_GR.
KEGG	acr:Acry_2675.
PATRIC	20650464. VBIAciCry6074_3191.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	DQERIEW.
ProtClustDB	PRK12558.
Enzyme and pathway databases
BioCyc	ACRY349163:ACRY_2675-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE2_ACICJ

Accession

Primary (citable) accession number: A5G1Y4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 12, 2007
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program