A5G1X4 (PURL_ACICJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	Acry_2665

Organism

Acidiphilium cryptum (strain JF-5) [Complete proteome] [HAMAP]

Taxonomic identifier

349163 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Acidiphilium

Protein attributes

Sequence length	727 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 727	727	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_1000050294
Regions
	Nucleotide binding	104 – 115	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A5G1X4 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: ACF57697293FD227	FASTA	727	75,946
	10 20 30 40 50 60 MNPTVIDAAL ARSFGLAAEE YDRVLAIMGR VPTLTELGIF SVMWSEHCSY KSSRIWLKTL 70 80 90 100 110 120 PTTAPWVIHG PGENAGVVDI GEGRAAIFKM ESHNHPSFIE PYQGAATGVG GILRDVFTMG 130 140 150 160 170 180 ARPVANLNAL RFGDPSLPVT KRVVDGVVRG IGGYGNCVGV PTVGGETNFH PSYNGNPLVN 190 200 210 220 230 240 AMTVGIADQD RIFLSAAAGI GNPVVYVGSK TGRDGIHGAT MASAEFGADA EEKRPTVQVG 250 260 270 280 290 300 DPFIEKLLIE ACLELMATDA IVAIQDMGAA GLTSSSVEMA GKGGVGIELD LDSVPQREAG 310 320 330 340 350 360 MSAYEMMLSE SQERMLIVLK PGREDMARAI FEKWELDFAV IGRITDSGRI VVKHQGRTEA 370 380 390 400 410 420 DIELAPLADQ APLYERPWVE PAKPAPLGPV ESPLPPGAAL LALIGCPDLA SRAWIWNQYD 430 440 450 460 470 480 GTVGGQTLRR PGQADAAIVR IEGSRRALAL TTDCTPRYCV ADPETGGAQA VVESWRNITA 490 500 510 520 530 540 TGATPLAVTD NLNFGNPEKP AIMGQIVAAI RGMGEACRAL DYPIVSGNVS LYNETEGTAI 550 560 570 580 590 600 LPTPAIGGLG VIDDGAAATG IALAPGLDLV LIGETSGELG ASLFLREILR REDGAPPALD 610 620 630 640 650 660 LAAEKRNGDF VRRQIHAGAI AACHDVSDGG LLVALAEMAL AGNTGIALAA IPEGIAPHAF 670 680 690 700 710 720 WFGEDQARYV AATADGAALI AEAAAAGIPA RPLGRSGGDH LTGPDGIAIS LSEIRAAHEA FFARWFA

References

[1]

"Complete sequence of chromosome of Acidiphilium cryptum JF-5." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Magnuson T., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JF-5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000697 Genomic DNA. Translation: ABQ31856.1.
RefSeq	YP_001235775.1. NC_009484.1.
3D structure databases
ProteinModelPortal	A5G1X4.
ModBase	Search...
Protein-protein interaction databases
STRING	A5G1X4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5161441.
GenomeReviews	Gene locus Acry_2665 in contig CP000697_GR.
KEGG	acr:Acry_2665.
PATRIC	20650446. VBIAciCry6074_3182.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0046.
HOGENOM	HBG311214.
OMA	YGNSFGV.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	ACRY349163:ACRY_2665-MONOMER.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_ACICJ

Accession

Primary (citable) accession number: A5G1X4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 12, 2007
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program