Reviewed, UniProtKB/Swiss-Prot A5G1L8 (ILVC_ACICJ)

Names and origin

Protein names

Recommended name:
    Ketol-acid reductoisomerase
    EC=1.1.1.86
Alternative name(s):
    Acetohydroxy-acid isomeroreductase
    Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	Acry_2559

Organism

Acidiphilium cryptum (strain JF-5) [Complete proteome] [HAMAP]

Taxonomic identifier

349163 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Acidiphilium

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Ketol-acid reductoisomerase		PRO_1000050471
Sites
	Active site	108	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	A5G1L8-1 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 115C767E96D37D42	FASTA	339	36,747
	10 20 30 40 50 60 MRVYYDSDAD VNLIKAKKVA VVGYGSQGHA HALNLKESGV KELVVALRKG SAAVAKAEAA 70 80 90 100 110 120 GLRVMTPEEA AAWADVVMIL TPDEGQGDLY RDSLAANLKP GAAIAFAHGL NIHFNLIEPR 130 140 150 160 170 180 ADIDVFMIAP KGPGHTVRSE YQRGGGVPCL VAVAQNPSGN ALDIALSYAS AIGGGRAGII 190 200 210 220 230 240 ETTFKEECET DLFGEQTVLC GGLVELIKAG FETLVEAGYA PEMAYFECLH EVKLIVDLIY 250 260 270 280 290 300 EGGIANMNYS ISNTAEYGEY VTGPRMITPE TKAEMKRVLD DIQKGRFTRD WMLENKVNQT 310 320 330 NFKAMRRANA AHPIEEVGEK LRAMMPWIKK GALVDKTRN

References

[1]

"Complete sequence of chromosome of Acidiphilium cryptum JF-5." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Magnuson T., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000697 Genomic DNA. Translation: ABQ31750.1.
RefSeq	YP_001235669.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5160054.
GenomeReviews	Gene locus Acry_2559 in contig CP000697_GR.
KEGG	acr:Acry_2559.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00435. [Tree]
InterPro	IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013116. IlvN. [Graphical view]
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_ACICJ

Accession

Primary (citable) accession number: A5G1L8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 12, 2007
Last modified:	November 25, 2008
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)