Reviewed, UniProtKB/Swiss-Prot A5G126 (SYD_ACICJ)

Names and origin

Protein names

Recommended name:
    Aspartyl-tRNA synthetase
    EC=6.1.1.12
Alternative name(s):
    Aspartate--tRNA ligase
      Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	Acry_2364

Organism

Acidiphilium cryptum (strain JF-5) [Complete proteome] [HAMAP]

Taxonomic identifier

349163 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Acidiphilium

Protein attributes

Sequence length	595 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044
Subunit structure	Homodimer By similarity. HAMAP MF_00044
Subcellular location	Cytoplasm By similarity HAMAP MF_00044.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 595	595	Aspartyl-tRNA synthetase HAMAP MF_00044		PRO_1000006626

Sequences

	Sequence		Length	Mass (Da)
	A5G126-1 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 8D629667057436C3	FASTA	595	64,858
	10 20 30 40 50 60 MHEYRTHDCG ALRAADAGIT ARLSGWVNVK RDHGGLLFID LRDHYGITQC VFPAGSPVFA 70 80 90 100 110 120 AAEALRAESV ITVTGRVVKR DADTVNPRLP TGEIELVAET VEVQSTADVL PIQVAGEAQF 130 140 150 160 170 180 PEELRLRYRF LDLRREKLHR NIMLRANVIA WLRREMTARG FTEFTTPILT ASSPEGARDF 190 200 210 220 230 240 LVPSRVHPGQ FYALPQAPQQ FKQLLMVAGF DRYFQIAPCF RDEASRADRA PGEFYQLDFE 250 260 270 280 290 300 MSFVTQEDVF ATIEPVLGGL FREFGGGRGV TQWPFPRIAY DEALLKYGSD KPDLRNPLLI 310 320 330 340 350 360 TDVTEAFRDS GFGLFAKIVA GGGVVRAIPA PGTGGNPRSF FDKLNDWARS EGAGGLGYII 370 380 390 400 410 420 FAPDGAKGPI AKNLEPARAE AIREATGASV GDAVFFAAGK PLEAAKFAGT VRTKLGTDLG 430 440 450 460 470 480 LIDGAKFEFC WIVDFPMYER DEETGQIVFS HNPFSMPQGG LEALNGQDPL TIKAFQYDIV 490 500 510 520 530 540 CNGVELSSGA IRNHRPDIML RAFEIAGYGA EEVEARFGGM LNAFRYGAPP HGGSAPGVDR 550 560 570 580 590 MVMLLADEPN LREVIAFPLN QQGQDLLMGA PAPVPAARLK ELSLAIALPP APKKG

References

[1]

"Complete sequence of chromosome of Acidiphilium cryptum JF-5." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Magnuson T., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000697 Genomic DNA. Translation: ABQ31558.1.
RefSeq	YP_001235477.1.
3D structure databases
SMR	A5G126. Positions 4-588.
ModBase	Search...
Protein-protein interaction databases
STRING	A5G126.
Genome annotation databases
GeneID	5160465.
GenomeReviews	Gene locus Acry_2364 in contig CP000697_GR.
KEGG	acr:Acry_2364.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0173.
HOGENOM	HBG396032.
OMA	YQLDVEM.
Family and domain databases
HAMAP	MF_00044_B. Asp_tRNA_synth_B. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-dom. IPR002312. Asp-tRNA-synth_IIb. IPR020564. Asp-tRNA-synth_IIb_bac-type. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR018153. Asp-tRNA-synth_IIb_C_bac/mt. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00459. aspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_ACICJ

Accession

Primary (citable) accession number: A5G126

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 12, 2007
Last modified:	February 9, 2010
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)