A5G0M4 (METE_ACICJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	Acry_2208

Organism

Acidiphilium cryptum (strain JF-5) [Complete proteome] [HAMAP]

Taxonomic identifier

349163 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Acidiphilium

Protein attributes

Sequence length	769 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 769	769	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000017214
Sites
	Metal binding	650	1	Zinc By similarity
	Metal binding	652	1	Zinc By similarity
	Metal binding	738	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5G0M4 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: C0A580C41034C5B6	FASTA	769	84,014
	10 20 30 40 50 60 MSIATSLGFP RIGRRRELKS ALEAHWAGEL SEAGLQEAAR LLRAESHSLQ QGLGIGHIPS 70 80 90 100 110 120 NDFALYDHVL DTACMVGAIP PGYGWRGGEV TLPTYFALAR GTGGGDAAAG LPALEMTKWF 130 140 150 160 170 180 DTNYHYLVPR LAAGQHFAVT ANRPLALFRE ALARHRRTRP VLLGPVSFLL LAKTDDGSDP 190 200 210 220 230 240 LDLLDRLLPC YAQVLAELAA EGCAWVQMDE PVLALDLAPK ARAALRHAYE TLARGATPRL 250 260 270 280 290 300 LLASYFAPIA DNLPTALALP VAGLHLDLVR GRDDLAPVLA AIGPATWLSL GLVDGRNVWR 310 320 330 340 350 360 ADLRAALATA REAARALGGS ERLMIAPSCS LLHVPVDLAQ EDRLDPAIRP WLAFATQKLA 370 380 390 400 410 420 EVATIARGLD EGEGAIAEAL EASDAALRTR RDSARVHRTD VAARLLGATP EMERRPAPHA 430 440 450 460 470 480 ARRARQRQRL PLPAFPTTTI GSLPQTSGVR RTRAALARGE IGAAEYDEAI ATWTEDAIRL 490 500 510 520 530 540 QERIGLDVLV HGEFERNDMV KYFGEQLDGF AFTRHGWVQS YGSRCVAPPI IWGDVARPRP 550 560 570 580 590 600 MTVRWARHAQ SLTARPVKGM LTGPVTMLQW SFVRDDLPRE TVCRQIALAL RDEVADLEAA 610 620 630 640 650 660 GLAIIQVDEP AFREGLPLRT ADREAYLRWA VSCFRLATAV VRDDTAIHTH MCYAEFQDIM 670 680 690 700 710 720 PAIATMDADA ISIETARSRM ELLEAFAGHG PSAYPAEIGP GVWDIHSPRI PPEEEILALL 730 740 750 760 RLARRKLADD QLWVNPDCGL KTRNWREVIP ALENLVGAAR RLRAEAIPA

References

[1]

"Complete sequence of chromosome of Acidiphilium cryptum JF-5." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Magnuson T., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JF-5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000697 Genomic DNA. Translation: ABQ31406.1.
RefSeq	YP_001235325.1. NC_009484.1.
3D structure databases
ProteinModelPortal	A5G0M4.
ModBase	Search...
Protein-protein interaction databases
STRING	A5G0M4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5159808.
GenomeReviews	Gene locus Acry_2208 in contig CP000697_GR.
KEGG	acr:Acry_2208.
PATRIC	20649496. VBIAciCry6074_2718.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0620.
HOGENOM	HBG287495.
OMA	RNIWRAN.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	ACRY349163:ACRY_2208-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_ACICJ

Accession

Primary (citable) accession number: A5G0M4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 12, 2007
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program