Reviewed, UniProtKB/Swiss-Prot A5G0L2 (SYT_ACICJ)

Names and origin

Protein names

Recommended name:
    Threonyl-tRNA synthetase
    EC=6.1.1.3
Alternative name(s):
    Threonine--tRNA ligase
      Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	Acry_2196

Organism

Acidiphilium cryptum (strain JF-5) [Complete proteome] [HAMAP]

Taxonomic identifier

349163 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Acidiphilium

Protein attributes

Sequence length	640 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00184
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP threonine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 640	640	Threonyl-tRNA synthetase HAMAP MF_00184		PRO_1000020330
Regions
	Region	243 – 536	294	Catalytic HAMAP MF_00184
Sites
	Metal binding	336	1	Zinc; catalytic By similarity
	Metal binding	387	1	Zinc; catalytic By similarity
	Metal binding	513	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5G0L2-1 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: E260B2C26F6807D9	FASTA	640	72,272
	10 20 30 40 50 60 MPAITLPDGS VRDYPGPVTG TEIAASIGPG LAKAALAMEV DGKMMDLSRT LDADARLRFI 70 80 90 100 110 120 TRRDADSLEL IRHDAAHVMA EAVQALYPGT QVTIGPAIEG GFYYDFFRNE PFTPDDLPAI 130 140 150 160 170 180 EAKMREIIAA NAPFTREVWD RDEAIRFFEA RGERFKAELI RDLPASETIT IYRQGDWLDL 190 200 210 220 230 240 CRGPHMRGTG DIGGAFKLTK VAGAYWRGDH RNPMLSRIYG TAWRDQKELD AHLKQLEEAE 250 260 270 280 290 300 RRDHRKIGRQ MDLFHMQEEA VGSVFWHEKG WRLYRTVEAY MRRRQEEFGY IEVRTPQLLD 310 320 330 340 350 360 RKLWEASGHW DNYRSHMFIA EVEDEDKVLA VKPMNCPCHV QIFNHGLRSY RELPLRMAEF 370 380 390 400 410 420 GACHRYEPSG ALHGIMRVRA FAQDDAHIFC TEDQIADETV RFVEMLRRVY ADFGFPEFAV 430 440 450 460 470 480 KFADRPTPRS GSDEVWDKAE AALRSACETA GVEFTVNPGE GAFYGPKLEF VLRDAIGRDW 490 500 510 520 530 540 QCGTLQVDFV LPERLDASYV TAESGRARPV MLHRAIVGSF ERFIGILIEN CAGRFPLWLA 550 560 570 580 590 600 PTQAAVASIV TDANDYAETV AARLKEAGLA VATDTRNEKI NAKVRDLSLA LVPNILVVGR 610 620 630 640 REAEQQTVSI RRLGSDKQDS MTLDEAIATL KAEATPPDLR

References

[1]

"Complete sequence of chromosome of Acidiphilium cryptum JF-5." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Magnuson T., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000697 Genomic DNA. Translation: ABQ31394.1.
RefSeq	YP_001235313.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A5G0L2.
Genome annotation databases
GeneID	5162563.
GenomeReviews	Gene locus Acry_2196 in contig CP000697_GR.
KEGG	acr:Acry_2196.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	QDEAPGM.
Family and domain databases
HAMAP	MF_00184. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR012675. b-grasp_ferredoxin-like. IPR004095. TGS. IPR002320. Thr-tRNA-synth_IIa. IPR018158. Thr-tRNA-synth_IIa_cons-reg. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
TIGRFAMs	TIGR00418. thrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_ACICJ

Accession

Primary (citable) accession number: A5G0L2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 12, 2007
Last modified:	November 3, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)