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A5G068 (SYL_ACICJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Acry_2050
OrganismAcidiphilium cryptum (strain JF-5) [Complete proteome] [HAMAP]
Taxonomic identifier349163 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeAcidiphilium

Protein attributes

Sequence length864 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 864864Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334723

Regions

Motif50 – 6011"HIGH" region HAMAP-Rule MF_00049
Motif622 – 6265"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6251ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A5G068 [UniParc].

Last modified June 12, 2007. Version 1.
Checksum: 59F41020AFB4B9E7

FASTA86495,028
        10         20         30         40         50         60 
MDQTTHDASA YDFTAAEARW QAAWEARNCF ATADAPQGGR RKCYVLEMFP YPSGKIHMGH 

        70         80         90        100        110        120 
VRNYAIGDVI ARARRAQGYD VLHPMGWDAF GLPAENAARE RNVDPAKWTR DNIAAMKADL 

       130        140        150        160        170        180 
KRVGLSVDWS REFATCDPEY YGHQQKLFLD LWRAGLAYRR ESAVNWDPVD MTVLANEQVI 

       190        200        210        220        230        240 
DGRGWKSGAP VEKRKLRQWF FRITDFAADL LAGLDTLENW PERVRTMQRN WIGRSEGAEF 

       250        260        270        280        290        300 
TIRLAAPCGG IESVPVYSTR PDTLFGMSFV ALAPDHPLAT AVAAANPEAA AFIAECQSAG 

       310        320        330        340        350        360 
TSEAAIEAAE KRGFDTGLRV VHPFDPSRTH PVWIANFVLM DYGTGAIFGC PAHDQRDLDF 

       370        380        390        400        410        420 
ARKYGLDVTV VVAPKDDPGL AVGDVAFTGD GVIVNSGFLD GLDVAAAKSR AIAELESRGA 

       430        440        450        460        470        480 
GKGVVNWRLR DWGVSRQRAW GCPIPMIHCE VCGTVPVPEK DLPVRLPDDL PFDRPGNALD 

       490        500        510        520        530        540 
HHPSWKHVAC PQCGAAAQRE TDTFDTFVDS SWYFARFCAP HAPVPADPAA TSHWMPVDHY 

       550        560        570        580        590        600 
IGGIEHAILH LLYARFFTRA MHRLGQVGVA EPFAGLFTQG MLTHESYRTE DGKWLYPEEV 

       610        620        630        640        650        660 
IRHADHATTL DGRKVIVGPI EKMSKSKRNT VDPSAVIARF GADTARWFVL SDNPPERDVE 

       670        680        690        700        710        720 
WTEAGAQGAF RYVQRLYRLA RSVAADRADD VALERAEGEA RKLRQATHRT IAAVTEAIDG 

       730        740        750        760        770        780 
FAFNVAIARL YELANAIAET EGRDAPGLPA ARREAMSALI RLAAPIIPHV AEEANAQVSP 

       790        800        810        820        830        840 
EAGLVVNQPW PVAEPELLKR DSVTLAVQIM GKLRGTIELP PGADAETAIA AAMAEPRIAQ 

       850        860 
LLEGATIVKR IHVPDRIVNF VVRP 

« Hide

References

[1]"Complete sequence of chromosome of Acidiphilium cryptum JF-5."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Kim E., Magnuson T., Richardson P.
Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JF-5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000697 Genomic DNA. Translation: ABQ31250.1.
RefSeqYP_001235169.1. NC_009484.1.

3D structure databases

ProteinModelPortalA5G068.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING349163.Acry_2050.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ31250; ABQ31250; Acry_2050.
GeneID5161511.
KEGGacr:Acry_2050.
PATRIC20649168. VBIAciCry6074_2555.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycACRY349163:GHET-2087-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_ACICJ
AccessionPrimary (citable) accession number: A5G068
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: June 12, 2007
Last modified: May 14, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries