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A5G043 (A5G043_ACICJ) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glutathione synthetase HAMAP-Rule MF_00162
EC=6.3.2.3 HAMAP-Rule MF_00162
Alternative name(s):
GSH synthetase HAMAP-Rule MF_00162
Glutathione synthase HAMAP-Rule MF_00162
Gene names
Name:gshB HAMAP-Rule MF_00162
Ordered Locus Names:Acry_2025 EMBL ABQ31225.1
OrganismAcidiphilium cryptum (strain JF-5) [Complete proteome] [HAMAP] EMBL ABQ31225.1
Taxonomic identifier349163 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Acidiphilium ›

Protein attributes

Sequence length317 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00162

Cofactor

Binds (magnesium or manganese,ion) per subunit By similarity. HAMAP-Rule MF_00162

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP-Rule MF_00162

Sequence similarities

Belongs to the prokaryotic GSH synthase family. HAMAP-Rule MF_00162

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00162

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain126 – 310185ATP-grasp By similarity HAMAP-Rule MF_00162
Nucleotide binding152 – 20857ATP By similarity HAMAP-Rule MF_00162

Sites

Metal binding2811Magnesium or manganese By similarity HAMAP-Rule MF_00162
Metal binding2831Magnesium or manganese By similarity HAMAP-Rule MF_00162

Sequences

Sequence LengthMass (Da)Tools
A5G043 [UniParc].

Last modified June 12, 2007. Version 1.
Checksum: 96611429E898D900

FASTA31735,276
        10         20         30         40         50         60 
MSGLRVAVQM DPMESVDIDG DSTFVLMLEA QRRGHALWHY TVRDMWLENG ALNARLRPVT 

        70         80         90        100        110        120 
VRREAGNHFA FGPAETMDLA AMDVVLMRQD PPFDMGYITA THLLEHIHPR TLVVNDPAAV 

       130        140        150        160        170        180 
RNAPEKLLVT DFPDLMPPTL IAWDRTAIRE FRARHKDIIV KPLYGNGGMG IFRIKPDDEN 

       190        200        210        220        230        240 
LGALLDTHFA GSREPLMVQR YEPAVRAGDK RIILIDGEPL GAINRVPAEG DARSNMHAGG 

       250        260        270        280        290        300 
IAEPTEMTAR DREICARIGP TLKERGLLFA GIDVIGDYLT EINVTSPTGL QQVARFNGLH 

       310 
LEATIWDRIE ARRASGH

« Hide

References

[1]"Complete sequence of chromosome of Acidiphilium cryptum JF-5."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Kim E., Magnuson T., Richardson P.
Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JF-5 EMBL ABQ31225.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000697 Genomic DNA. Translation: ABQ31225.1.
RefSeqYP_001235144.1. NC_009484.1.

3D structure databases

ProteinModelPortalA5G043.
ModBaseSearch...

Protein-protein interaction databases

STRING349163.Acry_2025.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ31225; ABQ31225; Acry_2025.
GeneID5160369.
KEGGacr:Acry_2025.
PATRIC20649116. VBIAciCry6074_2529.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0189.
HOGENOMHOG000265022.
KOK01920.
OMAWMRKDPP.
ProtClustDBPRK05246.

Enzyme and pathway databases

BioCycACRY349163:GHET-2079-MONOMER.
UniPathwayUPA00142; UER00210.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00162. GSH_S.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMsTIGR01380. glut_syn. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA5G043_ACICJ
AccessionPrimary (citable) accession number: A5G043
Entry history
Integrated into UniProtKB/TrEMBL: June 12, 2007
Last sequence update: June 12, 2007
Last modified: May 1, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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