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A5FZN9

- BIOB_ACICJ

UniProt

A5FZN9 - BIOB_ACICJ

Protein

Biotin synthase

Gene

bioB

Organism
Acidiphilium cryptum (strain JF-5)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (12 Jun 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi64 – 641Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi68 – 681Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi71 – 711Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi108 – 1081Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi139 – 1391Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi199 – 1991Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi271 – 2711Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciACRY349163:GHET-1903-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:Acry_1869
    OrganismiAcidiphilium cryptum (strain JF-5)
    Taxonomic identifieri349163 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeAcidiphilium
    ProteomesiUP000000245: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 325325Biotin synthasePRO_0000381176Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi349163.Acry_1869.

    Structurei

    3D structure databases

    ProteinModelPortaliA5FZN9.
    SMRiA5FZN9. Positions 16-321.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239957.
    KOiK01012.
    OMAiCKEDCIF.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A5FZN9-1 [UniParc]FASTAAdd to Basket

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    MSIALDSSTA TAAAAPSPTR ARIAALLALP LPELIFQAQT VHRQHFDPTQ    50
    VQISTLLSIK TGGCPEDCGY CPQSAEFDVG VKASKLMDIA PVLEAARRAK 100
    EAGAQRFCMG AAWRSPKDHD LDRVAAMVEG VKALGMETCV TLGMLRPDQA 150
    ARLKSAGLDY YNHNLDTSPE FYGSIITTRT YQDRLDTLAA VRDAGINICC 200
    GGIIGMGEDL DDRAGLLATL ADLDPPPESV PINALVAVKG TKLGESAPID 250
    PIDFVRTIAA ARISMPRSWV RLSAGREALS DEAQALCFLA GANSIFYGDT 300
    LLTTANPAVA RDNALFDRLG MTKLS 325
    Length:325
    Mass (Da):34,535
    Last modified:June 12, 2007 - v1
    Checksum:iA99AB5A63DD9BD77
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000697 Genomic DNA. Translation: ABQ31071.1.
    RefSeqiYP_001234990.1. NC_009484.1.

    Genome annotation databases

    EnsemblBacteriaiABQ31071; ABQ31071; Acry_1869.
    GeneIDi5159572.
    KEGGiacr:Acry_1869.
    PATRICi20648782. VBIAciCry6074_2364.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000697 Genomic DNA. Translation: ABQ31071.1 .
    RefSeqi YP_001234990.1. NC_009484.1.

    3D structure databases

    ProteinModelPortali A5FZN9.
    SMRi A5FZN9. Positions 16-321.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 349163.Acry_1869.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABQ31071 ; ABQ31071 ; Acry_1869 .
    GeneIDi 5159572.
    KEGGi acr:Acry_1869.
    PATRICi 20648782. VBIAciCry6074_2364.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239957.
    KOi K01012.
    OMAi CKEDCIF.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci ACRY349163:GHET-1903-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: JF-5.

    Entry informationi

    Entry nameiBIOB_ACICJ
    AccessioniPrimary (citable) accession number: A5FZN9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: June 12, 2007
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3