A5FZM1 (KATG_ACICJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	Acry_1851

Organism

Acidiphilium cryptum (strain JF-5) [Complete proteome] [HAMAP]

Taxonomic identifier

349163 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Acidiphilium

Protein attributes

Sequence length	728 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Domain	Signal
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 728	709	Catalase-peroxidase HAMAP MF_01961		PRO_0000354708
Sites
	Active site	97	1	Proton acceptor By similarity
	Metal binding	260	1	Iron (heme axial ligand) By similarity
	Site	93	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	96 ↔ 219		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-245) By similarity
	Cross-link	219 ↔ 245		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-96) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5FZM1 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 61E17724CD363C4D	FASTA	728	78,868
	10 20 30 40 50 60 MSTEAKCPVT GGATRSSSAG IQSNADWWPN QINLGMLHQH SALSNPMDPD FDYAAEFKTL 70 80 90 100 110 120 DLDAVIADLK ALMTDSQDWW PADFGHYGPL FVRMAWHAAG TYRIGDGRGG AGAGQQRFAP 130 140 150 160 170 180 LNSWPDNANL DKARRLLWPV KQKYGRKISW ADLMVLAGNV ALESMGFKTF GFGAGRVDTW 190 200 210 220 230 240 EPDQGIYWGP ETTWLDDKRY SGDRDLENPL AAVQMGLIYV NPEGPNGKPD PVAAARDIRE 250 260 270 280 290 300 TFARMAMNDE ETVALIAGGH TFGKTHGAGD AALVGPEPEA APIEQQGLGW ISSYGTGKGS 310 320 330 340 350 360 DAITGGPEVT WTQTPTQWSN FYFDNLFNYE WELTKSPAGA WQWVAKDAGD VIPDAFDAAK 370 380 390 400 410 420 KHRPTMLTTD LSMRMDPAYE KISRRFHQNP DEFADAFARA WFKLTHRDMG PVSRYLGKLV 430 440 450 460 470 480 PAEHLIWQDP VPAVDHKLID AADIAALKAK LLATGIAPTR LALTAWASAA TFRGSDKRGG 490 500 510 520 530 540 ANGARIRLAP QKDWAANEPA ELAKVLAALE KVQAEFNAAA TGGKKVSLAD LIVLGGCAAI 550 560 570 580 590 600 EAAAKAAGHD VTVPFTPGRT DATEAQTDVA SFAVLEPKAD GFRNYLGKGD PRAPEEQLID 610 620 630 640 650 660 RAQLMTLTAP EMTALIGGMR ALGANVGGAK HGVFTTRPGA LTNDFFVNLL DMNMSWHPAA 670 680 690 700 710 720 EPGVYELRDR KSGAVKWTAT RVDLVFGSNS QLRALAEVYG TQDGEAAFVK DFVAAWTKVM ELDRFDLA

References

[1]

"Complete sequence of chromosome of Acidiphilium cryptum JF-5." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Magnuson T., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JF-5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000697 Genomic DNA. Translation: ABQ31053.1.
RefSeq	YP_001234972.1. NC_009484.1.
3D structure databases
ProteinModelPortal	A5FZM1.
SMR	A5FZM1. Positions 23-728.
ModBase	Search...
Protein-protein interaction databases
STRING	A5FZM1.
Protein family/group databases
PeroxiBase	3597. AcrCP01_JF-5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5159721.
GenomeReviews	Gene locus Acry_1851 in contig CP000697_GR.
KEGG	acr:Acry_1851.
PATRIC	20648746. VBIAciCry6074_2346.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0376.
HOGENOM	HBG285610.
OMA	KRHAPSM.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	ACRY349163:ACRY_1851-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_ACICJ

Accession

Primary (citable) accession number: A5FZM1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	June 12, 2007
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program