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A5FZE9 (GLO2_ACICJ) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 32. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Hydroxyacylglutathione hydrolase
EC=3.1.2.6
Alternative name(s):
Glyoxalase II
Short name=Glx II
Gene names
Name:gloB
Ordered Locus Names:Acry_1778
OrganismAcidiphilium cryptum (strain JF-5) [Complete proteome] [HAMAP]
Taxonomic identifier349163 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Acidiphilium

Protein attributes

Sequence length243 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374

Catalytic activity

S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374

Cofactor

Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374

Pathway

Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374

Subunit structure

Monomer By similarity. HAMAP MF_01374

Sequence similarities

Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Ontologies

Keywords
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular functionhydroxyacylglutathione hydrolase activity

Inferred from electronic annotation. Source: EC

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 243243Hydroxyacylglutathione hydrolase HAMAP MF_01374
PRO_1000144747

Sites

Metal binding591Zinc 1 By similarity
Metal binding611Zinc 1 By similarity
Metal binding631Zinc 2 By similarity
Metal binding641Zinc 2 By similarity
Metal binding1171Zinc 1 By similarity
Metal binding1351Zinc 1 By similarity
Metal binding1351Zinc 2 By similarity
Metal binding1731Zinc 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A5FZE9 [UniParc].

Last modified June 12, 2007. Version 1.
Checksum: 9C74CDACA07C2B60

FASTA24325,336
        10         20         30         40         50         60 
MAISHRGFTI TRVPMLADNY AWLVAHGGAS AFVDPADAAA AIEAVEAAGG RLDWVLLTHH 

        70         80         90        100        110        120 
HDDHIAGAVD LAARFGARIA GNAADAARLP RLDAALAPGQ TIDLGGEAVR MIATPGHTVG 

       130        140        150        160        170        180 
HVTYLFGDAA AACGDTLFSL GCGRMFEGTP AQFHASLQAL AALDPETLML CGHEYTLSNA 

       190        200        210        220        230        240 
RFARHVDPDN AALAARAAEA ERLRAAGAPT LPVRLADELA ANPFLRAASA EEFARLRAAK 


DKF

« Hide

References

[1]"Complete sequence of chromosome of Acidiphilium cryptum JF-5."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Kim E., Magnuson T., Richardson P.
Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JF-5.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000697 Genomic DNA. Translation: ABQ30981.1.
RefSeqYP_001234900.1. NC_009484.1.

3D structure databases

ProteinModelPortalA5FZE9.
ModBaseSearch...

Protein-protein interaction databases

STRINGA5FZE9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5161578.
GenomeReviewsGene locus Acry_1778 in contig CP000697_GR.
KEGGacr:Acry_1778.
PATRIC20648598. VBIAciCry6074_2279.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0491.
HOGENOMHBG753931.
OMAIGCGRVF.

Enzyme and pathway databases

BioCycACRY349163:ACRY_1778-MONOMER.

Family and domain databases

HAMAPMF_01374. Glyoxalase_2.
[Tree]
InterProIPR001279. Beta-lactamas-like.
IPR017782. Hydroxyacylglutathione_Hdrlase.
[Graphical view]
KOK01069.
PANTHERPTHR11935:SF7. PTHR11935:SF7. 1 hit.
PfamPF00753. Lactamase_B. 1 hit.
[Graphical view]
SMARTSM00849. Lactamase_B. 1 hit.
[Graphical view]
TIGRFAMsTIGR03413. GSH_gloB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLO2_ACICJ
AccessionPrimary (citable) accession number: A5FZE9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: June 12, 2007
Last modified: January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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