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Protein

Chaperone protein DnaJ

Gene

dnaJ

Organism
Acidiphilium cryptum (strain JF-5)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi153Zinc 1UniRule annotation1
Metal bindingi156Zinc 1UniRule annotation1
Metal bindingi171Zinc 2UniRule annotation1
Metal bindingi174Zinc 2UniRule annotation1
Metal bindingi193Zinc 2UniRule annotation1
Metal bindingi196Zinc 2UniRule annotation1
Metal bindingi207Zinc 1UniRule annotation1
Metal bindingi210Zinc 1UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri140 – 219CR-typeUniRule annotationAdd BLAST80

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone
Biological processDNA replication, Stress response
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaJUniRule annotation
Gene namesi
Name:dnaJUniRule annotation
Ordered Locus Names:Acry_1645
OrganismiAcidiphilium cryptum (strain JF-5)
Taxonomic identifieri349163 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeAcidiphilium
Proteomesi
  • UP000000245 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001642331 – 383Chaperone protein DnaJAdd BLAST383

Interactioni

Subunit structurei

Homodimer.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

STRINGi349163.Acry_1645.

Structurei

3D structure databases

ProteinModelPortaliA5FZ18.
SMRiA5FZ18.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 70JUniRule annotationAdd BLAST66
Repeati153 – 160CXXCXGXG motif8
Repeati171 – 178CXXCXGXG motif8
Repeati193 – 200CXXCXGXG motif8
Repeati207 – 214CXXCXGXG motif8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi77 – 123Gly-richAdd BLAST47

Domaini

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.UniRule annotation

Sequence similaritiesi

Belongs to the DnaJ family.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri140 – 219CR-typeUniRule annotationAdd BLAST80

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG4105BZ5. Bacteria.
COG0484. LUCA.
HOGENOMiHOG000226717.
KOiK03686.
OMAiDMGGFAD.
OrthoDBiPOG091H00PT.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
cd10719. DnaJ_zf. 1 hit.
Gene3Di1.10.287.110. 1 hit.
HAMAPiMF_01152. DnaJ. 1 hit.
InterProiView protein in InterPro
IPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
PfamiView protein in Pfam
PF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
PRINTSiPR00625. JDOMAIN.
SMARTiView protein in SMART
SM00271. DnaJ. 1 hit.
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 4 hits.
SSF57938. SSF57938. 1 hit.
TIGRFAMsiTIGR02349. DnaJ_bact. 1 hit.
PROSITEiView protein in PROSITE
PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.

Sequencei

Sequence statusi: Complete.

A5FZ18-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKTDYYELL GVSRGASADE LKKAYRKLAM QYHPDRNPGD AAAEQKFKDI
60 70 80 90 100
NEAYDVLKDE QKRAAYDRFG HAAFENGGGP GAGGFGGGFG GFGGFEGGIG
110 120 130 140 150
DIFEQMFGEA MGGRRGGRTE RAGADLRAAV EIDLTQAFTG TKTEIRVPTR
160 170 180 190 200
VSCDACSGTG SADKSAANDT CPTCGGAGRV RAQQGFFVVE RTCPTCGGAG
210 220 230 240 250
RTVRNPCRVC SGAGTVPRER TLSVTIPAGV EDGTRIRLSG EGEAGGRGAS
260 270 280 290 300
PGDLYVHVSI RPHPIFQRDG ANVFCRVPLR MAQAALGGEI EVPAIDGTRA
310 320 330 340 350
RVKIPAGTQT GDQFRLRGKG FSVLRSTARG DMYIQVAVET PQNLTKRQRE
360 370 380
LLEEFEREAG NTASGSPEHE GFFAKVKEFF DGL
Length:383
Mass (Da):40,693
Last modified:June 12, 2007 - v1
Checksum:iC0F9AE1994557C0C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000697 Genomic DNA. Translation: ABQ30850.1.
RefSeqiWP_007421612.1. NC_009484.1.

Genome annotation databases

EnsemblBacteriaiABQ30850; ABQ30850; Acry_1645.
KEGGiacr:Acry_1645.

Similar proteinsi

Entry informationi

Entry nameiDNAJ_ACICJ
AccessioniPrimary (citable) accession number: A5FZ18
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 12, 2007
Last modified: June 7, 2017
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families