Reviewed, UniProtKB/Swiss-Prot A5FYS4 (PROA_ACICJ)

Names and origin

Protein names

Recommended name:
    Gamma-glutamyl phosphate reductase
      Short name=GPR
    EC=1.2.1.41
Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
      Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	Acry_1548

Organism

Acidiphilium cryptum (strain JF-5) [Complete proteome] [HAMAP]

Taxonomic identifier

349163 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Acidiphilium

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate By similarity.
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP(+) = L-glutamyl 5-phosphate + NADPH.
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	Gamma-glutamyl phosphate reductase		PRO_0000340869

Sequences

	Sequence		Length	Mass (Da)
	A5FYS4-1 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: F8347D4E4DC3D2A0	FASTA	420	43,953
	10 20 30 40 50 60 MDTATPSAEA ILRTAAGRAR AAMRSLAAMP AARRDGALRA AAAELRIHAV NILAANARDL 70 80 90 100 110 120 DSFEGPPAMR DRLLLNEARI EAIAAGIDAI ADLPDPLAGS LAEWTRPNGL VIRRVPQPLG 130 140 150 160 170 180 VIGMIYESRP NVGADAAAIC IKSGNAVILR GGSESYHSNT AIYLAMTKGL RQAGFPDGTV 190 200 210 220 230 240 QIAPNIDRAF VAAMLGASGQ IDLIIPRGGK SLVERVQREA RVPVLAHAEG LNHTYIHAEA 250 260 270 280 290 300 DAEMARTVLA NAKMRRTGIC GATETLLIDA AIAPALLPRL IEDLAALGCG FRADDRARAI 310 320 330 340 350 360 VPSLPAAGPA DFDTEWLDAM LSVSVVDDLD AALDHIARHG SSHTEAIVTE NADAAARFLA 370 380 390 400 410 420 GTDSAVAMWN ASTQFCDGGE FGFGAEIGIA TGRIHARGPI GPAQLTTFRY HVVGTGQVRP

References

[1]

"Complete sequence of chromosome of Acidiphilium cryptum JF-5." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Magnuson T., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000697 Genomic DNA. Translation: ABQ30756.1.
RefSeq	YP_001234675.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5161093.
GenomeReviews	Gene locus Acry_1548 in contig CP000697_GR.
KEGG	acr:Acry_1548.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00412. [Tree]
InterPro	IPR016162. Ald_DHase_N. IPR000965. Gglut_pp_reduct. IPR012134. Glu-5-SA_DHase. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
PIRSF	PIRSF000151. GPR. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_ACICJ

Accession

Primary (citable) accession number: A5FYS4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	June 12, 2007
Last modified:	November 25, 2008
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)