A5FYD2 (SYC_ACICJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	Acry_1404

Organism

Acidiphilium cryptum (strain JF-5) [Complete proteome] [HAMAP]

Taxonomic identifier

349163 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Acidiphilium

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00041
Subunit structure	Monomer By similarity. HAMAP MF_00041
Subcellular location	Cytoplasm HAMAP MF_00041.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 449	449	Cysteine--tRNA ligase HAMAP MF_00041		PRO_0000332777
Regions
	Motif	32 – 42	11	"HIGH" region HAMAP MF_00041
	Motif	268 – 272	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	30	1	Zinc By similarity
	Metal binding	210	1	Zinc By similarity
	Metal binding	235	1	Zinc By similarity
	Metal binding	239	1	Zinc By similarity
	Binding site	271	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5FYD2 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: A2DC6B6698FAA6A1	FASTA	449	48,863
	10 20 30 40 50 60 MIQIKLHNTK TRRREPFAPA DPAHVKLYVC GPTVYDRAHL GNARTVVVFD TLVRLLRHLF 70 80 90 100 110 120 PRVTYVRNIT DIDDKINARA AETGETIGEI TARTTSWFHE DMAALYCAPP DIEPRATGHI 130 140 150 160 170 180 GDIIALIERL IARGHAYAAE GHVLFAVATD AEYGKFSGRS PEELLAGARV DVATYKRDPG 190 200 210 220 230 240 DFVLWKPSPP DLPGWDSPWG RGRPGWHIEC SAMIHATLGE TIDIHGGGAD LIFPHHENEI 250 260 270 280 290 300 AQSCCAFPGS EFARVWVHAG MLQVDGQKMS KSLGNFRTVQ DVLGEAPGEA VRFLLLKTHY 310 320 330 340 350 360 RGVLDFSTAA LAEAKRELDR FYRALEKHAD PAPAATPPAA FIEALADDLN TPGAIAELHA 370 380 390 400 410 420 LADAAMQGDA ASAAGLRAAG MLIGIFNHTA DQWFRGEATD DARIDALIAE RLAARRNKDF 430 440 ARADAIRAEL AAAGILLEDG PGGTTWRRA

References

[1]

"Complete sequence of chromosome of Acidiphilium cryptum JF-5." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Magnuson T., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JF-5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000697 Genomic DNA. Translation: ABQ30614.1.
RefSeq	YP_001234533.1. NC_009484.1.
3D structure databases
ProteinModelPortal	A5FYD2.
ModBase	Search...
Protein-protein interaction databases
STRING	A5FYD2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5160514.
GenomeReviews	Gene locus Acry_1404 in contig CP000697_GR.
KEGG	acr:Acry_1404.
PATRIC	20647826. VBIAciCry6074_1901.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0215.
HOGENOM	HBG327651.
OMA	NVFDIHG.
ProtClustDB	PRK00260.
Enzyme and pathway databases
BioCyc	ACRY349163:ACRY_1404-MONOMER.
Family and domain databases
HAMAP	MF_00041. Cys_tRNA_synth. [Tree]
InterPro	IPR015803. Cys-tRNA-synt. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01883.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. CysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_ACICJ

Accession

Primary (citable) accession number: A5FYD2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	June 12, 2007
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program