Reviewed, UniProtKB/Swiss-Prot A5FXW5 (TPIS_ACICJ)

Names and origin

Protein names

Recommended name:
    Triosephosphate isomerase
      Short name=TIM
    EC=5.3.1.1
Alternative name(s):
    Triose-phosphate isomerase

Gene names

Name:	tpiA
Ordered Locus Names:	Acry_1236

Organism

Acidiphilium cryptum (strain JF-5) [Complete proteome] [HAMAP]

Taxonomic identifier

349163 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Acidiphilium

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate. HAMAP MF_00147
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00147 Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. HAMAP MF_00147
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis Pentose shunt
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: HAMAP glycolysis Inferred from electronic annotation. Source: HAMAP pentose-phosphate shunt Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	Triosephosphate isomerase HAMAP MF_00147		PRO_1000076633
Sites
	Active site	91	1	Electrophile By similarity
	Active site	168	1	Proton acceptor By similarity
	Binding site	8	1	Substrate By similarity
	Binding site	10	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5FXW5-1 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 68879343EE38EF8F	FASTA	253	25,333
	10 20 30 40 50 60 MPQLIAGNWK MNGTLAGIAA YAAALRPASP GAELLVCPPA PLIAPLRAAL DGAPVALGAQ 70 80 90 100 110 120 DCAVKRSGAH TGDLSADLLA ELGATHVILG HSERRADHAE SSATVREKAR TAIAAGLVPI 130 140 150 160 170 180 ICVGETEAER DSGEAETVVR AQLAGSLPEE LAGQTVPGVV APGIIAYEPV WAIGTGRTPT 190 200 210 220 230 240 EEDVAAMHAS IRAALRRQLG AAGATMPILY GGSVKPSNAA SLLALPEVGG ALVGGASLKA 250 EDFLAIASAA ARD

References

[1]

"Complete sequence of chromosome of Acidiphilium cryptum JF-5." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Magnuson T., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000697 Genomic DNA. Translation: ABQ30447.1.
RefSeq	YP_001234366.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A5FXW5.
Genome annotation databases
GeneID	5162402.
GenomeReviews	Gene locus Acry_1236 in contig CP000697_GR.
KEGG	acr:Acry_1236.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	IGAQDCH.
Family and domain databases
HAMAP	MF_00147. [Tree]
InterPro	IPR000652. Triosephosphate_isomerase. [Graphical view]
PANTHER	PTHR21139. Triophos_ismrse. 1 hit.
Pfam	PF00121. TIM. 1 hit. [Graphical view]
ProDom	PD001005. Triophos_ismrse. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00419. tim. 1 hit.
PROSITE	PS00171. TIM_1. 1 hit. PS51440. TIM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPIS_ACICJ

Accession

Primary (citable) accession number: A5FXW5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	June 12, 2007
Last modified:	November 3, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)