Reviewed, UniProtKB/Swiss-Prot A5FXD0 (ILVD_ACICJ)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase
      Short name=DAD
    EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	Acry_1046

Organism

Acidiphilium cryptum (strain JF-5) [Complete proteome] [HAMAP]

Taxonomic identifier

349163 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Acidiphilium

Protein attributes

Sequence length	617 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 617	617	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000000951
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	A5FXD0-1 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 560B4A9F02D7007A	FASTA	617	65,339
	10 20 30 40 50 60 MPQYRSRTST HGRNMAGARA LWRATGMGDA DFGKPIIAIA NSFTQFVPGH VHLKDLGQLV 70 80 90 100 110 120 AREIEAAGGV AKEFNTIAVD DGIAMGHGGM LYSLPSRELI ADAVEYMVNA HCADALVCIS 130 140 150 160 170 180 NCDKITPGML MAAMRLNIPT IFVSGGPMEA GKYIADGETR AADLITAMVV AADPTKTDEQ 190 200 210 220 230 240 AAVMERSACP TCGSCSGMFT ANSMNCLTEA LGLALPGNGS LLATHADRKR LFVEAGWQIV 250 260 270 280 290 300 DLARRYYEQD DEGVLPRRIG GFKAFENAMS LDIAMGGSTN TVLHLLAAAR EAELDFTMAD 310 320 330 340 350 360 IDRLSRRVPN LCKVSPSVSN VHMEDVHRAG GIMGILGALD RAGLIHRDCA TVHEKTIGEA 370 380 390 400 410 420 IDRWDVMRGG ETAKTLYSAA PGGVRTTEAF SQSRRYESLD LDREKGVIRD AEHAFSKDGG 430 440 450 460 470 480 LAVLYGNIAL DGAIVKTAGV DASILVFEGP ARIFESQEDA VAGILGDRVK AGDVVLIRYE 490 500 510 520 530 540 GPKGGPGMQE MLYPTSYLKS KGLGKSCALI TDGRFSGGTA GLSIGHISPE AAQGGAIGLV 550 560 570 580 590 600 EEGDIIAIDI PNRKLDVKLD EATLEARRAA MEAKGKAAWK PAARERVVSA ALQAYAALTT 610 SAANGAVRDV TQVQRGR

References

[1]

"Complete sequence of chromosome of Acidiphilium cryptum JF-5." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Magnuson T., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000697 Genomic DNA. Translation: ABQ30262.1.
RefSeq	YP_001234181.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A5FXD0.
Genome annotation databases
GeneID	5160237.
GenomeReviews	Gene locus Acry_1046 in contig CP000697_GR.
KEGG	acr:Acry_1046.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	SRKVPCL.
Family and domain databases
HAMAP	MF_00012. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
ProDom	PD002691. ILVD_EDD_family. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_ACICJ

Accession

Primary (citable) accession number: A5FXD0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 12, 2007
Last modified:	November 3, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)