Reviewed, UniProtKB/Swiss-Prot A5FXC2 (SYA_ACICJ)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Acry_1038

Organism

Acidiphilium cryptum (strain JF-5) [Complete proteome] [HAMAP]

Taxonomic identifier

349163 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Acidiphilium

Protein attributes

Sequence length	878 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 878	878	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000347468

Sequences

	Sequence		Length	Mass (Da)
	A5FXC2-1 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 1FC00F031C6DB515	FASTA	878	94,424
	10 20 30 40 50 60 MTSSNDIRAT FLDFFARNGH EVVPSAPLVP RNDPTLMFVN AGMVPFKNVF TGHEHRPYVR 70 80 90 100 110 120 AASSQKCVRA GGKHNDLDNV GYTARHHTFF EMLGNFSFGD YFKDVAIENA WTLLTRDFGL 130 140 150 160 170 180 PKEKLLVTVY HEDDEAASLW KRIAGLPDEK IIRIATDDNF WRMGDTGPCG PCSEIFYDHG 190 200 210 220 230 240 PSIPGGPPGS PDEDGDRFIE IWNLVFMQFL EEPAGVRNPL PRPSIDTGMG LERFAAILQG 250 260 270 280 290 300 KHDNYDTDTL RALILASAEV SHTDPDGAHK VSHRVIADHL RATSFLIADG VLPSNEGRGY 310 320 330 340 350 360 VLRRIMRRAM RHAHRLGMRE PFIYRLVPAL TRQMGVAFPE LPRAEALIVE TLKLEEQKFA 370 380 390 400 410 420 VMLERGLSLL EDEVQKLGSG EKLPGAVAFK LYDTYGFPLD LTEDALREQH RGLDHEGFTA 430 440 450 460 470 480 SMAEQKARAR AAWAGSGDAA TETVWLDLRD RLGGTEFLGY STEESDASIL AVVVNGAPAG 490 500 510 520 530 540 RAEAGQEVAL ILNQTPFYAE SGGQVGDTGT LTAPGVTIRV TDTQKKAGDL FVHYGTIEEG 550 560 570 580 590 600 EIAPGNSVHA EVDHARRGAI RAHHSATHLL HEALRRALGP HVTQKGSLNA PDRLRFDISQ 610 620 630 640 650 660 PRPVTGAELR AVEREVNARI RENAAVETRL MTPDEAVKRG AMALFGEKYG DEVRVVSMGA 670 680 690 700 710 720 GDAGRSAYSI ELCGGTHVAR TGDIGAFRIL AESGVAAGIR RIEAVTGEAA LAATEAETDL 730 740 750 760 770 780 LEEAAAALKV APAELPARIA ALLEEKKRFE RQVAELQRKL ATGGAAAEIE EVAGIKFAAR 790 800 810 820 830 840 NLGEVSPRDL KSLADSILKS MGSGIAALVS TAESKASIVV AVSPDLTSAN NAVTLVQAAS 850 860 870 GVVGGKGGGG RPDMAQAGGP DASKADEALA AVRALIKP

References

[1]

"Complete sequence of chromosome of Acidiphilium cryptum JF-5." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Magnuson T., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000697 Genomic DNA. Translation: ABQ30254.1.
RefSeq	YP_001234173.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5160168.
GenomeReviews	Gene locus Acry_1038 in contig CP000697_GR.
KEGG	acr:Acry_1038.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A5FXC2. GEFVIKL.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018165. Ala-tRNA-synth_IIc_cons-reg. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_ACICJ

Accession

Primary (citable) accession number: A5FXC2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	June 12, 2007
Last modified:	June 16, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)