A5FXC2 (SYA_ACICJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Acry_1038

Organism

Acidiphilium cryptum (strain JF-5) [Complete proteome] [HAMAP]

Taxonomic identifier

349163 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Acidiphilium

Protein attributes

Sequence length	878 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_B
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_B
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00036_B.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_B
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 878	878	Alanine--tRNA ligase HAMAP MF_00036_B		PRO_0000347468
Sites
	Metal binding	564	1	Zinc Potential
	Metal binding	568	1	Zinc Potential
	Metal binding	673	1	Zinc Potential
	Metal binding	677	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	A5FXC2 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 1FC00F031C6DB515	FASTA	878	94,424
	10 20 30 40 50 60 MTSSNDIRAT FLDFFARNGH EVVPSAPLVP RNDPTLMFVN AGMVPFKNVF TGHEHRPYVR 70 80 90 100 110 120 AASSQKCVRA GGKHNDLDNV GYTARHHTFF EMLGNFSFGD YFKDVAIENA WTLLTRDFGL 130 140 150 160 170 180 PKEKLLVTVY HEDDEAASLW KRIAGLPDEK IIRIATDDNF WRMGDTGPCG PCSEIFYDHG 190 200 210 220 230 240 PSIPGGPPGS PDEDGDRFIE IWNLVFMQFL EEPAGVRNPL PRPSIDTGMG LERFAAILQG 250 260 270 280 290 300 KHDNYDTDTL RALILASAEV SHTDPDGAHK VSHRVIADHL RATSFLIADG VLPSNEGRGY 310 320 330 340 350 360 VLRRIMRRAM RHAHRLGMRE PFIYRLVPAL TRQMGVAFPE LPRAEALIVE TLKLEEQKFA 370 380 390 400 410 420 VMLERGLSLL EDEVQKLGSG EKLPGAVAFK LYDTYGFPLD LTEDALREQH RGLDHEGFTA 430 440 450 460 470 480 SMAEQKARAR AAWAGSGDAA TETVWLDLRD RLGGTEFLGY STEESDASIL AVVVNGAPAG 490 500 510 520 530 540 RAEAGQEVAL ILNQTPFYAE SGGQVGDTGT LTAPGVTIRV TDTQKKAGDL FVHYGTIEEG 550 560 570 580 590 600 EIAPGNSVHA EVDHARRGAI RAHHSATHLL HEALRRALGP HVTQKGSLNA PDRLRFDISQ 610 620 630 640 650 660 PRPVTGAELR AVEREVNARI RENAAVETRL MTPDEAVKRG AMALFGEKYG DEVRVVSMGA 670 680 690 700 710 720 GDAGRSAYSI ELCGGTHVAR TGDIGAFRIL AESGVAAGIR RIEAVTGEAA LAATEAETDL 730 740 750 760 770 780 LEEAAAALKV APAELPARIA ALLEEKKRFE RQVAELQRKL ATGGAAAEIE EVAGIKFAAR 790 800 810 820 830 840 NLGEVSPRDL KSLADSILKS MGSGIAALVS TAESKASIVV AVSPDLTSAN NAVTLVQAAS 850 860 870 GVVGGKGGGG RPDMAQAGGP DASKADEALA AVRALIKP

References

[1]

"Complete sequence of chromosome of Acidiphilium cryptum JF-5." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Magnuson T., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JF-5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000697 Genomic DNA. Translation: ABQ30254.1.
RefSeq	YP_001234173.1. NC_009484.1.
3D structure databases
ProteinModelPortal	A5FXC2.
ModBase	Search...
Protein-protein interaction databases
STRING	A5FXC2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5160168.
GenomeReviews	Gene locus Acry_1038 in contig CP000697_GR.
KEGG	acr:Acry_1038.
PATRIC	20647076. VBIAciCry6074_1530.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0013.
HOGENOM	HBG354397.
OMA	GESKTDQ.
ProtClustDB	PRK00252.
Enzyme and pathway databases
BioCyc	ACRY349163:ACRY_1038-MONOMER.
Family and domain databases
HAMAP	MF_00036_B. Ala_tRNA_synth_B. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR023033. Ala_tRNA_synth_euk/bac. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_ACICJ

Accession

Primary (citable) accession number: A5FXC2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	June 12, 2007
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program