A5FX26 (SYP_ACICJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	Acry_0939

Organism

Acidiphilium cryptum (strain JF-5) [Complete proteome] [HAMAP]

Taxonomic identifier

349163 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Acidiphilium

Protein attributes

Sequence length	437 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP MF_01570
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01570
Subunit structure	Homodimer By similarity. HAMAP MF_01570
Subcellular location	Cytoplasm By similarity HAMAP MF_01570.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 437	437	Proline--tRNA ligase HAMAP MF_01570		PRO_1000069176

Sequences

	Sequence		Length	Mass (Da)
	A5FX26 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 6FED04E86BEB8430	FASTA	437	49,620
	10 20 30 40 50 60 MRLSRSLIPT LKETPAEAQI VSHRLMLRAG LIRQQSAGIY AWLPAGLRVL HNIANIVREE 70 80 90 100 110 120 QARAGSQEIL MPTIQSAELW RESGRYDAYG PEMLRIRDRH DREMLYGPTN EEMLTAIMRD 130 140 150 160 170 180 SVQSYRDLPQ MLYQIQWKFR DEVRPRFGVL RGREFYMKDG YSFDLDYEGA VESYRRMMLA 190 200 210 220 230 240 YMRTFKRMGV RAVPMRADTG PIGGNLSHEF HILAPTGESG VFYDSSFETI ELGDDAYDYE 250 260 270 280 290 300 ARADLDAFFD RMTSLYAATD EKHDEAAWAK VPEDRRREGR GIEVGQIFYF GTKYSQAMNF 310 320 330 340 350 360 TVVGPDGARL HPEMGSYGIG VSRLTGAIIE ASHDEAGIIW PDAIAPFRAS ILNLRQGDQV 370 380 390 400 410 420 TDALCERIYD ALGRDALYDD REARAGEKFA DADLMGHPWQ VIVGPRGAAK GQVELKHRRT 430 GERAELDIES ALAKVRV

References

[1]

"Complete sequence of chromosome of Acidiphilium cryptum JF-5." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Magnuson T., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JF-5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000697 Genomic DNA. Translation: ABQ30158.1.
RefSeq	YP_001234077.1. NC_009484.1.
3D structure databases
ProteinModelPortal	A5FX26.
SMR	A5FX26. Positions 1-435.
ModBase	Search...
Protein-protein interaction databases
STRING	A5FX26.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5161110.
GenomeReviews	Gene locus Acry_0939 in contig CP000697_GR.
KEGG	acr:Acry_0939.
PATRIC	20646872. VBIAciCry6074_1430.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0442.
HOGENOM	HBG403504.
OMA	IQPAELW.
ProtClustDB	PRK12325.
Enzyme and pathway databases
BioCyc	ACRY349163:ACRY_0939-MONOMER.
Family and domain databases
HAMAP	MF_01570. Pro_tRNA_synth_type2. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004500. Pro-tRNA-synth_IIa_bac-type. IPR023716. Prolyl-tRNA_Synthase_IIa_type2. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF3. PTHR11451:SF3. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SUPFAM	SSF52954. Anticodon_bd. 1 hit.
TIGRFAMs	TIGR00409. ProS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_ACICJ

Accession

Primary (citable) accession number: A5FX26

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	June 12, 2007
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program