Reviewed, UniProtKB/Swiss-Prot A5FWI5 (ASSY_ACICJ)

Names and origin

Protein names

Recommended name:
    Argininosuccinate synthase
    EC=6.3.4.5
Alternative name(s):
    Citrulline--aspartate ligase

Gene names

Name:	argG
Ordered Locus Names:	Acry_0747

Organism

Acidiphilium cryptum (strain JF-5) [Complete proteome] [HAMAP]

Taxonomic identifier

349163 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Acidiphilium

Protein attributes

Sequence length	409 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP MF_00005
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP MF_00005
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP argininosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 409	409	Argininosuccinate synthase HAMAP MF_00005		PRO_0000321296
Regions
	Nucleotide binding	12 – 20	9	ATP By similarity
Sites
	Binding site	39	1	ATP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	90	1	Citrulline By similarity
	Binding site	95	1	Citrulline By similarity
	Binding site	120	1	ATP; via amide nitrogen By similarity
	Binding site	122	1	Aspartate By similarity
	Binding site	126	1	Aspartate By similarity
	Binding site	126	1	Citrulline By similarity
	Binding site	127	1	Aspartate By similarity
	Binding site	130	1	Citrulline By similarity
	Binding site	181	1	Citrulline By similarity
	Binding site	190	1	Citrulline By similarity
	Binding site	266	1	Citrulline By similarity
	Binding site	278	1	Citrulline By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5FWI5-1 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: F4288A73438EE190	FASTA	409	44,926
	10 20 30 40 50 60 MAENSVKKVV LAYSGGLDTS VILRWLQTER GAEVVTFTAD LGQGEELEPA RRKAEMFGVK 70 80 90 100 110 120 EIFVEDLRET FVKDFVFPMF RANALYEGQY LLGTSIARPL IAQRQIEIAE AVGADAVAHG 130 140 150 160 170 180 ATGKGNDQVR FELAYYALKP DVKVVAPWRE WSLTSRTKLL EFAEAHQIPI AKDKRGEAPF 190 200 210 220 230 240 SVDANLLHSS SEGKILEDPA QAPDEIVYQR TISPEAAPDV VTEISIDFAQ GDAVALNGVA 250 260 270 280 290 300 LSPATLLTRL NELGRDNGIG RLDLVENRFV GMKSRGVYET PGGTILLAAH RAIESITLDR 310 320 330 340 350 360 EAAHLKDSLM PRYAELIYNG FWFSPERRML QALIDASQNS VTGRVRLKLY KGNVIVAGRE 370 380 390 400 SPNSLYSARM VTFEDDEGAY NQQDAAGFIK LNALRLRLGG AIGRRGGAL

References

[1]

"Complete sequence of chromosome of Acidiphilium cryptum JF-5." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Magnuson T., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000697 Genomic DNA. Translation: ABQ29967.1.
RefSeq	YP_001233886.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A5FWI5.
Genome annotation databases
GeneID	5159463.
GenomeReviews	Gene locus Acry_0747 in contig CP000697_GR.
KEGG	acr:Acry_0747.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	YVFPMFR.
Family and domain databases
HAMAP	MF_00005. [Tree]
InterPro	IPR001518. Arginosuc_synth. IPR018223. Arginosuc_synth_CS. [Graphical view]
PANTHER	PTHR11587. Arginosuc_synth. 1 hit.
Pfam	PF00764. Arginosuc_synth. 1 hit. [Graphical view]
TIGRFAMs	TIGR00032. argG. 1 hit.
PROSITE	PS00564. ARGININOSUCCIN_SYN_1. 1 hit. PS00565. ARGININOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASSY_ACICJ

Accession

Primary (citable) accession number: A5FWI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	June 12, 2007
Last modified:	November 3, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)