Reviewed, UniProtKB/Swiss-Prot A5FVZ7 (PANC_ACICJ)

Names and origin

Protein names

Recommended name:
    Pantothenate synthetase
      Short name=PS
    EC=6.3.2.1
Alternative name(s):
    Pantoate--beta-alanine ligase
    Pantoate-activating enzyme

Gene names

Name:	panC
Ordered Locus Names:	Acry_0555

Organism

Acidiphilium cryptum (strain JF-5) [Complete proteome] [HAMAP]

Taxonomic identifier

349163 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Acidiphilium

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate By similarity. HAMAP MF_00158
Catalytic activity	ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate. HAMAP MF_00158
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1. HAMAP MF_00158
Subunit structure	Homodimer By similarity. HAMAP MF_00158
Subcellular location	Cytoplasm Potential HAMAP MF_00158.
Miscellaneous	The reaction proceeds by a bi uni uni bi ping pong mechanism By similarity. HAMAP MF_00158
Sequence similarities	Belongs to the pantothenate synthetase family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW pantoate-beta-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 278	278	Pantothenate synthetase HAMAP MF_00158		PRO_1000118137
Regions
	Nucleotide binding	31 – 38	8	ATP By similarity
	Nucleotide binding	148 – 151	4	ATP By similarity
	Nucleotide binding	185 – 188	4	ATP By similarity
Sites
	Active site	38	1	Proton donor By similarity
	Binding site	62	1	Beta-alanine By similarity
	Binding site	62	1	Pantoate By similarity
	Binding site	154	1	Pantoate By similarity
	Binding site	177	1	ATP; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5FVZ7-1 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 2951F2019399BB31	FASTA	278	29,641
	10 20 30 40 50 60 MQIVRNVPDL RAARAALTDG GRARLALVPT MGALHEGHLS LVRAAREAGH VVAASIFVNP 70 80 90 100 110 120 TQFGPNEDFT RYPRDPERDC ALLAEAGCAL AWLPEVSTMY PPGDATSVEV AGLSVVLEGA 130 140 150 160 170 180 VRPGHYRGVA TVVVKLLGQV RPDAAYFGEK DWQQVQVIRR VAADLFLPVE IVVGPTLREK 190 200 210 220 230 240 DGLAMSSRNR YLDPADRARA PLLHQVLRRV RAALRDGEPA AAALAAGRRA LAEGGFIVDY 250 260 270 LELVDAGTLA PLTAPDDGAR LLAAARLGTT RLLDTVAV

References

[1]

"Complete sequence of chromosome of Acidiphilium cryptum JF-5." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Magnuson T., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000697 Genomic DNA. Translation: ABQ29779.1.
RefSeq	YP_001233698.1.
3D structure databases
SMR	A5FVZ7. Positions 1-278.
ModBase	Search...
Protein-protein interaction databases
STRING	A5FVZ7.
Genome annotation databases
GeneID	5161487.
GenomeReviews	Gene locus Acry_0555 in contig CP000697_GR.
KEGG	acr:Acry_0555.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0414.
HOGENOM	HBG428839.
OMA	MPIQIIG.
Family and domain databases
HAMAP	MF_00158. PanC. [Tree]
InterPro	IPR003721. Pantoate_ligase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR21299:SF1. Pantoate_ligase. 1 hit.
Pfam	PF02569. Pantoate_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00018. panC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANC_ACICJ

Accession

Primary (citable) accession number: A5FVZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	June 12, 2007
Last modified:	February 9, 2010
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)