A5FV65 (FABH_ACICJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-oxoacyl-[acyl-carrier-protein] synthase 3
EC=2.3.1.41

Alternative name(s):
3-oxoacyl-[acyl-carrier-protein] synthase III
Beta-ketoacyl-ACP synthase III
Short name=KAS III

Gene names

Name:	fabH
Ordered Locus Names:	Acry_0269

Organism

Acidiphilium cryptum (strain JF-5) [Complete proteome] [HAMAP]

Taxonomic identifier

349163 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Acidiphilium

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched-chain and/or straight-chain of fatty acids By similarity. HAMAP MF_01815
Catalytic activity	Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein]. HAMAP MF_01815
Pathway	Lipid metabolism; fatty acid biosynthesis. HAMAP MF_01815
Subunit structure	Homodimer By similarity. HAMAP MF_01815
Subcellular location	Cytoplasm Probable HAMAP MF_01815.
Domain	The last Arg residue of the ACP-binding site is essential for the weak association between ACP/AcpP and FabH By similarity. HAMAP MF_01815
Sequence similarities	Belongs to the FabH family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-oxoacyl-[acyl-carrier-protein] synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	3-oxoacyl-[acyl-carrier-protein] synthase 3 HAMAP MF_01815		PRO_1000070211
Regions
	Region	248 – 252	5	ACP-binding By similarity
Sites
	Active site	113	1	By similarity
	Active site	247	1	By similarity
	Active site	277	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5FV65 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 32703E5D412C5367	FASTA	320	33,874
	10 20 30 40 50 60 MQRTVLEGVG SYLPERIVTN HELATRIDTS DAWIRERTGI GQRHIAAAHE TATFMGAEAA 70 80 90 100 110 120 RRALAAAGAS ADSVDAVIVA TSTPDQGFPA TAVSIQAAIG MTRGFAFDLS AACSGFVYGV 130 140 150 160 170 180 SVADAMIRAG QCRSALVIGT EVYSRILNWE DRGTCVLFGD GAGAVLLRAG TGEDDRGVIS 190 200 210 220 230 240 THIHSDGRYG DILFIDGATG QDDRPQHLVM NGREVFRHAV SKLAGAVDEA LAANDLTQAD 250 260 270 280 290 300 IDWLVPHQAN RRIIDAMGKR LGLAPEQVVV TVDRHANTSA ASIPLALDEA VRDGRIRRGH 310 320 LVLIEALGGG LTWGSALIRF

References

[1]

"Complete sequence of chromosome of Acidiphilium cryptum JF-5." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Magnuson T., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JF-5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000697 Genomic DNA. Translation: ABQ29497.1.
RefSeq	YP_001233416.1. NC_009484.1.
3D structure databases
ProteinModelPortal	A5FV65.
SMR	A5FV65. Positions 5-320.
ModBase	Search...
Protein-protein interaction databases
STRING	A5FV65.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5160307.
GenomeReviews	Gene locus Acry_0269 in contig CP000697_GR.
KEGG	acr:Acry_0269.
PATRIC	20645490. VBIAciCry6074_0749.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0332.
HOGENOM	HBG649927.
OMA	TVAIHAN.
ProtClustDB	CLSK935911.
Enzyme and pathway databases
BioCyc	ACRY349163:ACRY_0269-MONOMER.
Family and domain databases
HAMAP	MF_01815. FabH. [Tree]
InterPro	IPR013751. ACP_syn_III. IPR013747. ACP_syn_III_C. IPR004655. FabH_synth. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 2 hits.
KO	K00648.
Pfam	PF08545. ACP_syn_III. 1 hit. PF08541. ACP_syn_III_C. 1 hit. [Graphical view]
SUPFAM	SSF53901. Thiolase-like. 1 hit.
TIGRFAMs	TIGR00747. FabH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FABH_ACICJ

Accession

Primary (citable) accession number: A5FV65

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	June 12, 2007
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program