A5FV42 (EFG_ACICJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor G
Short name=EF-G

Gene names

Name:	fusA
Ordered Locus Names:	Acry_0246

Organism

Acidiphilium cryptum (strain JF-5) [Complete proteome] [HAMAP]

Taxonomic identifier

349163 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Acidiphilium

Protein attributes

Sequence length	695 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome By similarity. HAMAP MF_00054_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00054_B.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 695	695	Elongation factor G HAMAP MF_00054_B		PRO_1000008797
Regions
	Nucleotide binding	16 – 23	8	GTP By similarity
	Nucleotide binding	80 – 84	5	GTP By similarity
	Nucleotide binding	134 – 137	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5FV42 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 1E8F4806B90E1B1B	FASTA	695	76,649
	10 20 30 40 50 60 MSGTSLAKIR NIGITAHIDA GKTTTTERIL YYTGVSHKIG EVHDGNTTTD YMEQERERGI 70 80 90 100 110 120 TITSAAVTCE WKDHRINIID TPGHIDFNIE VNRSLRVLDG AVFIIEGVAG VQPQSETNWR 130 140 150 160 170 180 LADRYNVPRI IFINKLDRTG ADFYRAFDTL KEKLDIVALP LQLPIGIEDQ FVGVVDLVEM 190 200 210 220 230 240 KAIVWEGGEL GAKFHDEEIP ADMLEKAKEY RQNLLDTALS VDDAGMEEYF EKGDVATETL 250 260 270 280 290 300 KRAIKTGTIS GAFRPVLCGT AFKNKGVQPL LDAVLDYLPS PVDIEGIKVA PPEGEEEDPS 310 320 330 340 350 360 ANRRVIPANP DAPFAGLAFK IINDKYGTLT FVRVYAGTLR SGDTVLNTTK GHKERVGRMF 370 380 390 400 410 420 QMHADKREEV KEVHAGDIAA FVGLKDTGTG DTLASSDDPV VLERMAFPVP VIDISVEPKT 430 440 450 460 470 480 KEAVEKMTLA LQKLAGEDPS LRLKTDQETG QTILSGMGEL HLDIIIDRLR REYGVEANVG 490 500 510 520 530 540 APQVAYRETI TREHTETYTH KKQSGGSGQF AEVKIIFEPQ ERNEGILFEN KVVGGAVPKE 550 560 570 580 590 600 YIPAVEKGIK VQADTGVLAG FPTVDFKYTL VDGKYHDVDS SALAFEIAAK ACFREGMKKA 610 620 630 640 650 660 GPVILEPIMD VEITTPQDHV GDVVGDLNRR RGMIQNQESS GSTVIVRAQV PLKEMFGYIS 670 680 690 NLRSMTKGRA SFTMQFHHYD PVPRNIADEI MTKSA

References

[1]

"Complete sequence of chromosome of Acidiphilium cryptum JF-5." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Magnuson T., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JF-5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000697 Genomic DNA. Translation: ABQ29474.1.
RefSeq	YP_001233393.1. NC_009484.1.
3D structure databases
ProteinModelPortal	A5FV42.
ModBase	Search...
Protein-protein interaction databases
STRING	A5FV42.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5160984.
GenomeReviews	Gene locus Acry_0246 in contig CP000697_GR.
KEGG	acr:Acry_0246.
PATRIC	20645440. VBIAciCry6074_0725.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0480.
HOGENOM	HBG737692.
OMA	CNEWREK.
ProtClustDB	PRK12739.
Enzyme and pathway databases
BioCyc	ACRY349163:ACRY_0246-MONOMER.
Family and domain databases
HAMAP	MF_00054_B. EF-G_EF-2_B. [Tree]
InterPro	IPR009022. Elongation_fac_G/III/V. IPR000795. ProtSyn_GTP-bd. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR005225. Small_GTP-bd_dom. IPR004540. Transl_elong_EFG/EF2. IPR000640. Transl_elong_EFG/EF2_C. IPR005517. Transl_elong_EFG/EF2_IV. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. G3DSA:3.30.70.240. Transl_elong_EFG/EF2_C. 1 hit.
KO	K02355.
Pfam	PF00679. EFG_C. 1 hit. PF03764. EFG_IV. 1 hit. PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SMART	SM00838. EFG_C. 1 hit. SM00889. EFG_IV. 1 hit. [Graphical view]
SUPFAM	SSF54980. EFG_III_V. 2 hits. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00484. EF-G. 1 hit. TIGR00231. Small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFG_ACICJ

Accession

Primary (citable) accession number: A5FV42

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 12, 2007
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program