Reviewed, UniProtKB/Swiss-Prot A5FUL2 (MURB_ACICJ)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylenolpyruvoylglucosamine reductase
    EC=1.1.1.158
Alternative name(s):
    UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	Acry_0065

Organism

Acidiphilium cryptum (strain JF-5) [Complete proteome] [HAMAP]

Taxonomic identifier

349163 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Acidiphilium

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037		PRO_1000002859
Regions
	Domain	34 – 198	165	FAD-binding PCMH-type
Sites
	Active site	178	1	By similarity
	Active site	227	1	Proton donor By similarity
	Active site	297	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5FUL2-1 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: FF401F5CCBCA0E18	FASTA	309	32,424
	10 20 30 40 50 60 MMAAPVMADW RAALPEVRGR IGFDVPLGPV TWFRVGGPAE VMFRPADIED LSRFLAALAP 70 80 90 100 110 120 EVPVLPIGAA SNLIVRDGGI AGVVVRLVRG FADIEVQPDG IVAGAAALDA TIAEHAAAAG 130 140 150 160 170 180 LTGLEFLSGI PGSLGGAVAM NAGAYGAEIR DVLDWAEIVG RDGTVARYAA GDLALTYRHA 190 200 210 220 230 240 RLPEGGIVVR ARLHARPGEA AAIAARMADI RASREATQPV RARTGGSTFR NPEGDKAWRL 250 260 270 280 290 300 IDEAGCRGLI HGGAQVSEKH CNFLINLGEA TAADIEGLGE TVRRRVRERT GVELIWEIRR VGLPCRGAA

References

[1]

"Complete sequence of chromosome of Acidiphilium cryptum JF-5." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Magnuson T., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000697 Genomic DNA. Translation: ABQ29294.1.
RefSeq	YP_001233213.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A5FUL2.
Genome annotation databases
GeneID	5160152.
GenomeReviews	Gene locus Acry_0065 in contig CP000697_GR.
KEGG	acr:Acry_0065.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	QPIREKT.
Family and domain databases
HAMAP	MF_00037. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00179. murB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB_ACICJ

Accession

Primary (citable) accession number: A5FUL2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 12, 2007
Last modified:	November 3, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)