Reviewed, UniProtKB/Swiss-Prot A5FUL1 (MURC_ACICJ)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	Acry_0064

Organism

Acidiphilium cryptum (strain JF-5) [Complete proteome] [HAMAP]

Taxonomic identifier

349163 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Acidiphilium

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 479	479	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_1000004303
Regions
	Nucleotide binding	115 – 121	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A5FUL1-1 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: DFAAA16FCD1CB187	FASTA	479	51,126
	10 20 30 40 50 60 MRAMPLSIGT IHFVGIAGIG MSGIAEVLHN LGYSVQGSDL SENANVARLR AAGIPVAIGH 70 80 90 100 110 120 DAANLGNAQV VVVSTAVPRD NPEVQAARKR MIPVVRRAEM LGELMRLRWS VAIGGTHGKT 130 140 150 160 170 180 TTTSLVAAVL EGAGLDPTVI NGGIINAYGT NARLGGGDWM VVEADESDGS FLRLPAVIAV 190 200 210 220 230 240 VTNMDPEHLD HWGSAEAMEA GYRQFVSNIP FYGFAVLCID HPGVQRMIPD LSDHRLITYG 250 260 270 280 290 300 LSPQADVRAE RIMSDRNGAT FEVRLSERVA GRERVLAPMR LPMLGNHNVQ NALAAIAIGI 310 320 330 340 350 360 EMEVPEVDLR AALASFRGVK RRFTKTGEVA GITVIDDYGH HPVEIAAVLR AARQAGARDV 370 380 390 400 410 420 IAVVQPHRYT RLATLFEDFC TCMNDAGKVI VADVYPAGEE PIPGIDRDAL VEGLRARGHK 430 440 450 460 470 SVVSLGSPDH LAEMINAMAR AGDYVVCLGA GSITNWAQAL PNQLQALIET TRRGAGGMR

References

[1]

"Complete sequence of chromosome of Acidiphilium cryptum JF-5." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Magnuson T., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000697 Genomic DNA. Translation: ABQ29293.1.
RefSeq	YP_001233212.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A5FUL1.
Genome annotation databases
GeneID	5160151.
GenomeReviews	Gene locus Acry_0064 in contig CP000697_GR.
KEGG	acr:Acry_0064.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	EWMVVEA.
Family and domain databases
HAMAP	MF_00046. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_ACICJ

Accession

Primary (citable) accession number: A5FUL1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 12, 2007
Last modified:	November 3, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)