Reviewed, UniProtKB/Swiss-Prot A5FUK7 (MRAY_ACICJ)

Names and origin

Protein names

Recommended name:
    Phospho-N-acetylmuramoyl-pentapeptide-transferase
    EC=2.7.8.13
Alternative name(s):
    UDP-MurNAc-pentapeptide phosphotransferase

Gene names

Name:	mraY
Ordered Locus Names:	Acry_0060

Organism

Acidiphilium cryptum (strain JF-5) [Complete proteome] [HAMAP]

Taxonomic identifier

349163 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Acidiphilium

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan By similarity. HAMAP MF_00038
Catalytic activity	UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol. HAMAP MF_00038
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00038
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_00038.
Sequence similarities	Belongs to the glycosyltransferase 4 family. MraY subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	phospho-N-acetylmuramoyl-pentapeptide-transferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 362	362	Phospho-N-acetylmuramoyl-pentapeptide-transferase HAMAP MF_00038		PRO_0000332523
Regions
	Transmembrane	21 – 41	21	Potential
	Transmembrane	75 – 95	21	Potential
	Transmembrane	100 – 120	21	Potential
	Transmembrane	136 – 156	21	Potential
	Transmembrane	170 – 190	21	Potential
	Transmembrane	201 – 221	21	Potential
	Transmembrane	247 – 267	21	Potential
	Transmembrane	290 – 310	21	Potential
	Transmembrane	339 – 359	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	A5FUK7-1 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 1201B95D4AA18706	FASTA	362	39,023
	10 20 30 40 50 60 MLYALLLPHV AAFHAFNLIR YITFRAGGAC LTALVVSFLL GPRLIRWLKS LQKQGQPIRA 70 80 90 100 110 120 DGPERHLIEK KGTPTMGGFL ILIALTVSTL LWADLRNGYV WAVLMITIGY GALGFADDFL 130 140 150 160 170 180 KLTKRNTKGV PGRIKLVVQA VLGLGAAVWI TQLMPGSIAD SLAVPVFKHL MIPFGPLFPL 190 200 210 220 230 240 VAMFVMMGAS NAVNLTDGLD GLAIVPTIIA AGVFTLIAYL VGNRIFSHYL EINFVPGTGE 250 260 270 280 290 300 LAVFCSALIG AGMGFLWFNA PPAAVFMGDT GSLALGGALG SVAVATKNEI VLAITGGLFV 310 320 330 340 350 360 VETVSVIVQV FWYKRTGRRV FLMAPLHHHF EKKGWAEPTV VIRFWIVAMI LALLGLATLK IR

References

[1]

"Complete sequence of chromosome of Acidiphilium cryptum JF-5." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Magnuson T., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000697 Genomic DNA. Translation: ABQ29289.1.
RefSeq	YP_001233208.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A5FUK7.
Genome annotation databases
GeneID	5160779.
GenomeReviews	Gene locus Acry_0060 in contig CP000697_GR.
KEGG	acr:Acry_0060.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0472.
HOGENOM	HBG708263.
OMA	HHHFEQK.
Family and domain databases
HAMAP	MF_00038. MraY. [Tree]
InterPro	IPR000715. Glycosyl_transferase_4. IPR018481. Glycosyl_Trfase_4_cons-reg. IPR003524. PNAcMuramoyl-5peptid_Trfase. IPR018480. PNAcMuramoyl-5peptid_Trfase_CS. [Graphical view]
PANTHER	PTHR22926. Glyco_trans_4. 1 hit. PTHR22926:SF3. PNAcPpept_trans. 1 hit.
Pfam	PF00953. Glycos_transf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00445. mraY. 1 hit.
PROSITE	PS01347. MRAY_1. False negative. PS01348. MRAY_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MRAY_ACICJ

Accession

Primary (citable) accession number: A5FUK7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	June 12, 2007
Last modified:	February 9, 2010
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)