A5FRB9 (DXS_DEHSB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	DehaBAV1_0675

Organism

Dehalococcoides sp. (strain BAV1) [Complete proteome] [HAMAP]

Taxonomic identifier

216389 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Dehalococcoidetes › Dehalococcoides

Protein attributes

Sequence length	633 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 633	633	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_1000119543

Sequences

	Sequence		Length	Mass (Da)
	A5FRB9 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: BCDCA8DDB704BFEE	FASTA	633	68,581
	10 20 30 40 50 60 MSKLLDTINS PSDLKKLSLD ELRELAVQIR EELVNRVTLN GGHLASSLGV VELTIALHRV 70 80 90 100 110 120 FESPKDKIIW DVGHQSYAHK LLTGRREQFA TLRQHGGLSG FTCRDESPHD PFGAGHASTS 130 140 150 160 170 180 ISAGLGMAVA RDLAKEDYSV ISVIGDGAIS GGMSFEAINN AGHLHTKFIV ILNDNGMAIS 190 200 210 220 230 240 PSTGALSKFL NNVRFDPRFE FAKRNAKQTI TNMPFGKAVW AFTKSIKRKF EKSMLPGSLW 250 260 270 280 290 300 EELGFIYLGP VDGHNIRELE AALKRAKDFE SKPVLIHMIT KKGKGYDDAE ADAVKYHGIS 310 320 330 340 350 360 PKSGGLKSSH GLSYSQVFGQ TLHKIMSQNP QVVAITAAMT DGCGLGEIAA AFPDRVFDVG 370 380 390 400 410 420 ICEQHAVTFA AGMATQGYIP VVVIYSTFLQ RGFDQIIHDV CLQKLPVVFA IDRGGIVGDD 430 440 450 460 470 480 GKTHQGIFDL SFMSLIPDMV VSAPSDENDL QHLLYTAVNS GKPFALRYPR GFGEGVEIES 490 500 510 520 530 540 SLHNIPIGQN EILVNGSDVA ILATGKSVAF AREALEILTE SGIKPTLVNN RYISPLDSEL 550 560 570 580 590 600 VLKIAQSHKY LVTVEENVIS GGLGSRINTL LAEAGLVNKI KIANIGIPDK FVEHGNQSLL 610 620 630 RAKYGLDGKG IAQRVLSLVG NPNEMKHPQI ICP

References

[1]

"Complete sequence of Dehalococcoides sp. BAV1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Lowry S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Ritalahti K.M., Loeffler F., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BAV1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000688 Genomic DNA. Translation: ABQ17259.1.
RefSeq	YP_001214137.1. NC_009455.1.
3D structure databases
ProteinModelPortal	A5FRB9.
ModBase	Search...
Protein-protein interaction databases
STRING	A5FRB9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5131286.
GenomeReviews	Gene locus DehaBAV1_0675 in contig CP000688_GR.
KEGG	deb:DehaBAV1_0675.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1154.
HOGENOM	HBG571647.
OMA	QYDISYM.
ProtClustDB	PRK05444.
Enzyme and pathway databases
BioCyc	DSP216389:DEHABAV1_0675-MONOMER.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR001017. DH_E1. IPR005477. Dxylulose-5-P_synthase. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF00676. E1_dh. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_DEHSB

Accession

Primary (citable) accession number: A5FRB9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	June 12, 2007
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program