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Protein

tRNA dimethylallyltransferase

Gene

miaA

Organism
Dehalococcoides mccartyi (strain ATCC BAA-2100 / JCM 16839 / KCTC 5957 / BAV1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i6A).UniRule annotation

Catalytic activityi

Dimethylallyl diphosphate + adenine(37) in tRNA = diphosphate + N(6)-dimethylallyladenine(37) in tRNA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi25 – 328ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciDSP216389:GH6D-686-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA dimethylallyltransferaseUniRule annotation (EC:2.5.1.75UniRule annotation)
Alternative name(s):
Dimethylallyl diphosphate:tRNA dimethylallyltransferaseUniRule annotation
Short name:
DMAPP:tRNA dimethylallyltransferaseUniRule annotation
Short name:
DMATaseUniRule annotation
Isopentenyl-diphosphate:tRNA isopentenyltransferaseUniRule annotation
Short name:
IPP transferaseUniRule annotation
Short name:
IPPTUniRule annotation
Short name:
IPTaseUniRule annotation
Gene namesi
Name:miaAUniRule annotation
Ordered Locus Names:DehaBAV1_0671
OrganismiDehalococcoides mccartyi (strain ATCC BAA-2100 / JCM 16839 / KCTC 5957 / BAV1)
Taxonomic identifieri216389 [NCBI]
Taxonomic lineageiBacteriaChloroflexiDehalococcoidiaDehalococcoidalesDehalococcoidaceaeDehalococcoides

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 328328tRNA dimethylallyltransferasePRO_0000377138Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei116 – 1161Interaction with substrate tRNAUniRule annotation
Sitei139 – 1391Interaction with substrate tRNAUniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA5FRB5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni27 – 326Substrate bindingUniRule annotation
Regioni50 – 534Interaction with substrate tRNAUniRule annotation

Sequence similaritiesi

Belongs to the IPP transferase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000039995.
KOiK00791.
OMAiHTRNYAK.
OrthoDBiEOG661HB3.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00174. miaA. 1 hit.

Sequencei

Sequence statusi: Complete.

A5FRB5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIIDSTTGA NASNLPLSPL LVILGNTGSG KSALAINLAK VIPAGIINAD
60 70 80 90 100
SRQIYSRMDI ATAKPTLSEM LEIPHYLYSF IQPDQPFSLA EYQTLAYQAI
110 120 130 140 150
DSLHTQNRLP VLVGGSGQYL KAVLEGWSIP PVAPDETFRA ALFEKAEKEG
160 170 180 190 200
GDVIFTELEK KDPQAATQID PRNIRRVVRA LEVIHKTGGK FSGLLIKNPP
210 220 230 240 250
PYRVFKIGIH IPREELYRTV DLRVEKMLEQ GLEAEVRSLL EKGYKASLPS
260 270 280 290 300
MSGIGYRQIA KYIEGGISLT EAVEQMKFET HRLIRQQNTY FRLTDTNIHW
310 320
ITLEESRSNG IIQLVCDFLA AENKNEIH
Length:328
Mass (Da):36,574
Last modified:June 12, 2007 - v1
Checksum:i239F3357F01376C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000688 Genomic DNA. Translation: ABQ17255.1.
RefSeqiWP_011929009.1. NC_009455.1.

Genome annotation databases

EnsemblBacteriaiABQ17255; ABQ17255; DehaBAV1_0671.
KEGGideb:DehaBAV1_0671.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000688 Genomic DNA. Translation: ABQ17255.1.
RefSeqiWP_011929009.1. NC_009455.1.

3D structure databases

ProteinModelPortaliA5FRB5.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ17255; ABQ17255; DehaBAV1_0671.
KEGGideb:DehaBAV1_0671.

Phylogenomic databases

HOGENOMiHOG000039995.
KOiK00791.
OMAiHTRNYAK.
OrthoDBiEOG661HB3.

Enzyme and pathway databases

BioCyciDSP216389:GH6D-686-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00174. miaA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-2100 / JCM 16839 / KCTC 5957 / BAV1.

Entry informationi

Entry nameiMIAA_DEHMB
AccessioniPrimary (citable) accession number: A5FRB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: June 12, 2007
Last modified: November 11, 2015
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.