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Protein

tRNA dimethylallyltransferase

Gene

miaA

Organism
Dehalococcoides mccartyi (strain ATCC BAA-2100 / JCM 16839 / KCTC 5957 / BAV1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i6A).UniRule annotation

Catalytic activityi

Dimethylallyl diphosphate + adenine(37) in tRNA = diphosphate + N6-dimethylallyladenine(37) in tRNA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi25 – 32ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processtRNA processing
LigandATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciDMCC216389:G1G8W-705-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA dimethylallyltransferaseUniRule annotation (EC:2.5.1.75UniRule annotation)
Alternative name(s):
Dimethylallyl diphosphate:tRNA dimethylallyltransferaseUniRule annotation
Short name:
DMAPP:tRNA dimethylallyltransferaseUniRule annotation
Short name:
DMATaseUniRule annotation
Isopentenyl-diphosphate:tRNA isopentenyltransferaseUniRule annotation
Short name:
IPP transferaseUniRule annotation
Short name:
IPPTUniRule annotation
Short name:
IPTaseUniRule annotation
Gene namesi
Name:miaAUniRule annotation
Ordered Locus Names:DehaBAV1_0671
OrganismiDehalococcoides mccartyi (strain ATCC BAA-2100 / JCM 16839 / KCTC 5957 / BAV1)
Taxonomic identifieri216389 [NCBI]
Taxonomic lineageiBacteriaChloroflexiDehalococcoidiaDehalococcoidalesDehalococcoidaceaeDehalococcoides

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003771381 – 328tRNA dimethylallyltransferaseAdd BLAST328

Proteomic databases

PRIDEiA5FRB5

Interactioni

Subunit structurei

Monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei116Interaction with substrate tRNAUniRule annotation1
Sitei139Interaction with substrate tRNAUniRule annotation1

Structurei

3D structure databases

ProteinModelPortaliA5FRB5
SMRiA5FRB5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni27 – 32Substrate bindingUniRule annotation6
Regioni50 – 53Interaction with substrate tRNAUniRule annotation4

Sequence similaritiesi

Belongs to the IPP transferase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000039995
KOiK00791
OMAiGTGLYIR

Family and domain databases

HAMAPiMF_00185 IPP_trans, 1 hit
InterProiView protein in InterPro
IPR018022 IPT
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF01715 IPPT, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00174 miaA, 1 hit

Sequencei

Sequence statusi: Complete.

A5FRB5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIIDSTTGA NASNLPLSPL LVILGNTGSG KSALAINLAK VIPAGIINAD
60 70 80 90 100
SRQIYSRMDI ATAKPTLSEM LEIPHYLYSF IQPDQPFSLA EYQTLAYQAI
110 120 130 140 150
DSLHTQNRLP VLVGGSGQYL KAVLEGWSIP PVAPDETFRA ALFEKAEKEG
160 170 180 190 200
GDVIFTELEK KDPQAATQID PRNIRRVVRA LEVIHKTGGK FSGLLIKNPP
210 220 230 240 250
PYRVFKIGIH IPREELYRTV DLRVEKMLEQ GLEAEVRSLL EKGYKASLPS
260 270 280 290 300
MSGIGYRQIA KYIEGGISLT EAVEQMKFET HRLIRQQNTY FRLTDTNIHW
310 320
ITLEESRSNG IIQLVCDFLA AENKNEIH
Length:328
Mass (Da):36,574
Last modified:June 12, 2007 - v1
Checksum:i239F3357F01376C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000688 Genomic DNA Translation: ABQ17255.1
RefSeqiWP_011929009.1, NC_009455.1

Genome annotation databases

EnsemblBacteriaiABQ17255; ABQ17255; DehaBAV1_0671
KEGGideb:DehaBAV1_0671
PATRICifig|216389.18.peg.720

Similar proteinsi

Entry informationi

Entry nameiMIAA_DEHMB
AccessioniPrimary (citable) accession number: A5FRB5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: June 12, 2007
Last modified: May 23, 2018
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

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