A5FRA7 (DEF_DEHSB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptide deformylase
Short name=PDF
EC=3.5.1.88

Alternative name(s):
Polypeptide deformylase

Gene names

Name:	def
Ordered Locus Names:	DehaBAV1_0687

Organism

Dehalococcoides sp. (strain BAV1) [Complete proteome] [HAMAP]

Taxonomic identifier

216389 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Dehalococcoidetes › Dehalococcoides

Protein attributes

Sequence length	167 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity. HAMAP MF_00163
Catalytic activity	Formyl-L-methionyl peptide + H2O = formate + methionyl peptide. HAMAP MF_00163
Cofactor	Binds 1 Fe2+ ion By similarity. HAMAP MF_00163
Sequence similarities	Belongs to the polypeptide deformylase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	Iron Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	iron ion binding Inferred from electronic annotation. Source: InterPro peptide deformylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 167	167	Peptide deformylase HAMAP MF_00163		PRO_1000076942
Sites
	Active site	133	1	By similarity
	Metal binding	90	1	Iron By similarity
	Metal binding	132	1	Iron By similarity
	Metal binding	136	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5FRA7 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: BEB2EF2613623500	FASTA	167	18,448
	10 20 30 40 50 60 MAIRRICELP EPVLRKKAKK VPSIDGSIQT LIDDMIETMN SADGAGLAAP QVGVSLRLVV 70 80 90 100 110 120 FREPDTKEAT VLINPEIIKK EGQRQVTEGC LSIPGYFGEL TRAETVTAKG LDRHGKACRI 130 140 150 160 KGTGIVAQLL EHETEHLDGI LYIDHLESED QLHEIGPDDE MPEEIRE

References

[1]

"Complete sequence of Dehalococcoides sp. BAV1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Lowry S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Ritalahti K.M., Loeffler F., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BAV1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000688 Genomic DNA. Translation: ABQ17271.1.
RefSeq	YP_001214149.1. NC_009455.1.
3D structure databases
ProteinModelPortal	A5FRA7.
ModBase	Search...
Protein-protein interaction databases
STRING	A5FRA7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5132326.
GenomeReviews	Gene locus DehaBAV1_0687 in contig CP000688_GR.
KEGG	deb:DehaBAV1_0687.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0242.
HOGENOM	HBG665227.
OMA	VWEPIGE.
ProtClustDB	PRK00150.
Enzyme and pathway databases
BioCyc	DSP216389:DEHABAV1_0687-MONOMER.
Family and domain databases
HAMAP	MF_00163. Pep_deformylase. [Tree]
InterPro	IPR000181. Fmet_deformylase. IPR023635. Peptide_deformylase. [Graphical view]
Gene3D	G3DSA:3.90.45.10. Fmet_deformylase. 1 hit.
KO	K01462.
PANTHER	PTHR10458. Fmet_deformylase. 1 hit.
Pfam	PF01327. Pep_deformylase. 1 hit. [Graphical view]
PIRSF	PIRSF004749. Pep_def. 1 hit.
PRINTS	PR01576. PDEFORMYLASE.
SUPFAM	SSF56420. Fmet_deformylase. 1 hit.
TIGRFAMs	TIGR00079. Pept_deformyl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DEF_DEHSB

Accession

Primary (citable) accession number: A5FRA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	June 12, 2007
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program