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Protein

Ribonuclease PH

Gene

rph

Organism
Dehalococcoides mccartyi (strain ATCC BAA-2100 / JCM 16839 / KCTC 5957 / BAV1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Phosphorolytic 3'-5' exoribonuclease that plays an important role in tRNA 3'-end maturation. Removes nucleotide residues following the 3'-CCA terminus of tRNAs; can also add nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates, but this may not be physiologically important. Probably plays a role in initiation of 16S rRNA degradation (leading to ribosome degradation) during starvation.UniRule annotation

Catalytic activityi

tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei87Phosphate (substrate) bindingUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processrRNA processing, tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease PHUniRule annotation (EC:2.7.7.56UniRule annotation)
Short name:
RNase PHUniRule annotation
Alternative name(s):
tRNA nucleotidyltransferaseUniRule annotation
Gene namesi
Name:rphUniRule annotation
Ordered Locus Names:DehaBAV1_1100
OrganismiDehalococcoides mccartyi (strain ATCC BAA-2100 / JCM 16839 / KCTC 5957 / BAV1)
Taxonomic identifieri216389 [NCBI]
Taxonomic lineageiBacteriaChloroflexiDehalococcoidiaDehalococcoidalesDehalococcoidaceaeDehalococcoides

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000822891 – 241Ribonuclease PHAdd BLAST241

Proteomic databases

PRIDEiA5FQ49

Interactioni

Subunit structurei

Homohexameric ring arranged as a trimer of dimers.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA5FQ49
SMRiA5FQ49
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni125 – 127Phosphate (substrate) bindingUniRule annotation3

Sequence similaritiesi

Belongs to the RNase PH family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000229516
KOiK00989
OMAiKGKGQGW

Family and domain databases

CDDicd11362 RNase_PH_bact, 1 hit
Gene3Di3.30.230.70, 1 hit
HAMAPiMF_00564 RNase_PH, 1 hit
InterProiView protein in InterPro
IPR001247 ExoRNase_PH_dom1
IPR015847 ExoRNase_PH_dom2
IPR036345 ExoRNase_PH_dom2_sf
IPR027408 PNPase/RNase_PH_dom_sf
IPR020568 Ribosomal_S5_D2-typ_fold
IPR002381 RNase_PH_bac-type
IPR018336 RNase_PH_CS
PfamiView protein in Pfam
PF01138 RNase_PH, 1 hit
PF03725 RNase_PH_C, 1 hit
SUPFAMiSSF54211 SSF54211, 1 hit
SSF55666 SSF55666, 1 hit
TIGRFAMsiTIGR01966 RNasePH, 1 hit
PROSITEiView protein in PROSITE
PS01277 RIBONUCLEASE_PH, 1 hit

Sequencei

Sequence statusi: Complete.

A5FQ49-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQRGDGRNFN QLRPITITPG FQSFAEGSVL IEQGKTRVIC SVSMEDRVPQ
60 70 80 90 100
FLRNSGTGWV TAEYSMLPRS TVTRTQRDSS AGKISGRSQE IQRLIGRSLR
110 120 130 140 150
SCVDMAALGE RSFIVDCDVI QADAGTRTAS ITGSYIALYL AFKKMVDMGI
160 170 180 190 200
LSKMPFTSQV AAVSVSIFKG NIVLDPCYDE DFQAEVDFNL VMNDRGEFVE
210 220 230 240
IQGTAEGKAF SRDTLDQVLK LGEAGIRQLF DIQKGITQPQ L
Length:241
Mass (Da):26,556
Last modified:June 12, 2007 - v1
Checksum:iE7EDE4931AA9EDEC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000688 Genomic DNA Translation: ABQ17680.1
RefSeqiWP_011309647.1, NC_009455.1

Genome annotation databases

EnsemblBacteriaiABQ17680; ABQ17680; DehaBAV1_1100
GeneIDi29935766
KEGGideb:DehaBAV1_1100
PATRICifig|216389.18.peg.1162

Similar proteinsi

Entry informationi

Entry nameiRNPH_DEHMB
AccessioniPrimary (citable) accession number: A5FQ49
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: June 12, 2007
Last modified: May 23, 2018
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

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