Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A5FP73 (SYL_DEHSB) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:DehaBAV1_0159
OrganismDehalococcoides sp. (strain BAV1) [Complete proteome] [HAMAP]
Taxonomic identifier216389 [NCBI]
Taxonomic lineageBacteriaChloroflexiDehalococcoidiaDehalococcoidalesDehalococcoidaceaeDehalococcoides

Protein attributes

Sequence length813 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 813813Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000199193

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif580 – 5845"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5831ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A5FP73 [UniParc].

Last modified June 12, 2007. Version 1.
Checksum: FC5F0958E48100CB

FASTA81392,816
        10         20         30         40         50         60 
MAEKYNPQET EKKWQDKWAA DRLYHASEDS PKPKWYSLTM FPYTSGNLHI GHWYAEVPAD 

        70         80         90        100        110        120 
CFARYKRLRG FNVMRPVGFD SFGLPAENAA IKHNIHPRIW TLNNVENMRR QLKTIGAMFD 

       130        140        150        160        170        180 
WDREVITCLP EYYKWTQWFF LKLYEAGLAY RAKAPVNWCP SCQAVLANEQ VVDGTCWRCE 

       190        200        210        220        230        240 
TPTTRRDLEQ WFFRITNYAD ELKDHEGLDW PEKITAMQRN WVGKSYGAEV SFALDCPTAF 

       250        260        270        280        290        300 
EKEIKVFTTR PDTIYGVTFM VLAPEHPLVE KITTPENKAA VDAYIKKSRT CTEIERLSTE 

       310        320        330        340        350        360 
REKDGVFTGT YVTNRVNGQK VPVWIGDYVL QSYGTGAVMG VPAHDERDFV FAQKYHLPVI 

       370        380        390        400        410        420 
TVIAPSAYDG KPLEAAYINE GVMLNSGPFN GTPNTEGKEK VCDYLAEHGW GKKTVNYKLR 

       430        440        450        460        470        480 
DWLISRQRYW GAPIPMVYCE KCGIVPVPEK DLPVLLPEDV GFRSGGESPL KYNEGFVNTI 

       490        500        510        520        530        540 
CPVCGGKAKR ETDTMDTFMC SSWYFLRYTS PGYDKGPFDP VKLKYWMPVD LYTGGAEHAV 

       550        560        570        580        590        600 
MHLFYSRFFT KALRDMGIID FGEPFKKLFN QGIIVSNHQK MSKSKGNVVT PDNLVAEVGT 

       610        620        630        640        650        660 
DAVRAYLMFV GPWDQGGEWN DSGLSGMSRW LNRVWNLFTE EYTPQTASAE AERELKRTLH 

       670        680        690        700        710        720 
QTIKKITMDI ERLRFNTVVA ALMELSNSLA KLKETAAISA ENWQNTLQTF ALMLAPVAPH 

       730        740        750        760        770        780 
IAEELWANLG MKYSIHNQNW PTWDEELAKD EVITLIIQVN GKLRERLEMP AGISEAEAKE 

       790        800        810 
TALNSERVKP HLLGKTPVTV IYVPGKLVNI VVK 

« Hide

References

[1]"Complete sequence of Dehalococcoides sp. BAV1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Lowry S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Ritalahti K.M., Loeffler F., Richardson P.
Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BAV1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000688 Genomic DNA. Translation: ABQ16750.1.
RefSeqYP_001213628.1. NC_009455.1.

3D structure databases

ProteinModelPortalA5FP73.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING216389.DehaBAV1_0159.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ16750; ABQ16750; DehaBAV1_0159.
GeneID5132037.
KEGGdeb:DehaBAV1_0159.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycDSP216389:GH6D-162-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYL_DEHSB
AccessionPrimary (citable) accession number: A5FP73
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 12, 2007
Last modified: April 16, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries