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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Dehalococcoides mccartyi (strain ATCC BAA-2100 / JCM 16839 / KCTC 5957 / BAV1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei583 – 5831ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciDSP216389:GH6D-162-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:DehaBAV1_0159
OrganismiDehalococcoides mccartyi (strain ATCC BAA-2100 / JCM 16839 / KCTC 5957 / BAV1)
Taxonomic identifieri216389 [NCBI]
Taxonomic lineageiBacteriaChloroflexiDehalococcoidiaDehalococcoidalesDehalococcoidaceaeDehalococcoides
ProteomesiUP000002607 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 813813Leucine--tRNA ligasePRO_1000199193Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi216389.DehaBAV1_0159.

Structurei

3D structure databases

ProteinModelPortaliA5FP73.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi580 – 5845"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

A5FP73-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEKYNPQET EKKWQDKWAA DRLYHASEDS PKPKWYSLTM FPYTSGNLHI
60 70 80 90 100
GHWYAEVPAD CFARYKRLRG FNVMRPVGFD SFGLPAENAA IKHNIHPRIW
110 120 130 140 150
TLNNVENMRR QLKTIGAMFD WDREVITCLP EYYKWTQWFF LKLYEAGLAY
160 170 180 190 200
RAKAPVNWCP SCQAVLANEQ VVDGTCWRCE TPTTRRDLEQ WFFRITNYAD
210 220 230 240 250
ELKDHEGLDW PEKITAMQRN WVGKSYGAEV SFALDCPTAF EKEIKVFTTR
260 270 280 290 300
PDTIYGVTFM VLAPEHPLVE KITTPENKAA VDAYIKKSRT CTEIERLSTE
310 320 330 340 350
REKDGVFTGT YVTNRVNGQK VPVWIGDYVL QSYGTGAVMG VPAHDERDFV
360 370 380 390 400
FAQKYHLPVI TVIAPSAYDG KPLEAAYINE GVMLNSGPFN GTPNTEGKEK
410 420 430 440 450
VCDYLAEHGW GKKTVNYKLR DWLISRQRYW GAPIPMVYCE KCGIVPVPEK
460 470 480 490 500
DLPVLLPEDV GFRSGGESPL KYNEGFVNTI CPVCGGKAKR ETDTMDTFMC
510 520 530 540 550
SSWYFLRYTS PGYDKGPFDP VKLKYWMPVD LYTGGAEHAV MHLFYSRFFT
560 570 580 590 600
KALRDMGIID FGEPFKKLFN QGIIVSNHQK MSKSKGNVVT PDNLVAEVGT
610 620 630 640 650
DAVRAYLMFV GPWDQGGEWN DSGLSGMSRW LNRVWNLFTE EYTPQTASAE
660 670 680 690 700
AERELKRTLH QTIKKITMDI ERLRFNTVVA ALMELSNSLA KLKETAAISA
710 720 730 740 750
ENWQNTLQTF ALMLAPVAPH IAEELWANLG MKYSIHNQNW PTWDEELAKD
760 770 780 790 800
EVITLIIQVN GKLRERLEMP AGISEAEAKE TALNSERVKP HLLGKTPVTV
810
IYVPGKLVNI VVK
Length:813
Mass (Da):92,816
Last modified:June 11, 2007 - v1
Checksum:iFC5F0958E48100CB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000688 Genomic DNA. Translation: ABQ16750.1.
RefSeqiWP_011928671.1. NC_009455.1.
YP_001213628.1. NC_009455.1.

Genome annotation databases

EnsemblBacteriaiABQ16750; ABQ16750; DehaBAV1_0159.
KEGGideb:DehaBAV1_0159.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000688 Genomic DNA. Translation: ABQ16750.1.
RefSeqiWP_011928671.1. NC_009455.1.
YP_001213628.1. NC_009455.1.

3D structure databases

ProteinModelPortaliA5FP73.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi216389.DehaBAV1_0159.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ16750; ABQ16750; DehaBAV1_0159.
KEGGideb:DehaBAV1_0159.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciDSP216389:GH6D-162-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-2100 / JCM 16839 / KCTC 5957 / BAV1.

Entry informationi

Entry nameiSYL_DEHMB
AccessioniPrimary (citable) accession number: A5FP73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 27, 2009
Last sequence update: June 11, 2007
Last modified: March 31, 2015
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.