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Protein

Kynureninase

Gene

kynU

Organism
Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / UW101) (Cytophaga johnsonae)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively.UniRule annotation

Catalytic activityi

L-kynurenine + H2O = anthranilate + L-alanine.UniRule annotation
L-3-hydroxykynurenine + H2O = 3-hydroxyanthranilate + L-alanine.UniRule annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotation

Pathwayi: L-kynurenine degradation

This protein is involved in step 1 of the subpathway that synthesizes L-alanine and anthranilate from L-kynurenine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Kynureninase (kynU)
This subpathway is part of the pathway L-kynurenine degradation, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-alanine and anthranilate from L-kynurenine, the pathway L-kynurenine degradation and in Amino-acid degradation.

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes quinolinate from L-kynurenine.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Kynurenine 3-monooxygenase (kmo)
  2. Kynureninase (kynU)
  3. 3-hydroxyanthranilate 3,4-dioxygenase (nbaC)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes quinolinate from L-kynurenine, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei105Pyridoxal phosphate; via amide nitrogenUniRule annotation1
Binding sitei106Pyridoxal phosphateUniRule annotation1
Binding sitei218Pyridoxal phosphateUniRule annotation1
Binding sitei221Pyridoxal phosphateUniRule annotation1
Binding sitei243Pyridoxal phosphateUniRule annotation1
Binding sitei274Pyridoxal phosphateUniRule annotation1
Binding sitei302Pyridoxal phosphateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00253; UER00329.
UPA00334; UER00455.

Names & Taxonomyi

Protein namesi
Recommended name:
KynureninaseUniRule annotation (EC:3.7.1.3UniRule annotation)
Alternative name(s):
L-kynurenine hydrolaseUniRule annotation
Gene namesi
Name:kynUUniRule annotation
Ordered Locus Names:Fjoh_0506
OrganismiFlavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / UW101) (Cytophaga johnsonae)
Taxonomic identifieri376686 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesFlavobacteriiaFlavobacterialesFlavobacteriaceaeFlavobacterium
Proteomesi
  • UP000006694 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003570051 – 425KynureninaseAdd BLAST425

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei244N6-(pyridoxal phosphate)lysineUniRule annotation1

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi376686.Fjoh_0506.

Structurei

3D structure databases

ProteinModelPortaliA5FMM4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni133 – 136Pyridoxal phosphate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the kynureninase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CKY. Bacteria.
COG3844. LUCA.
HOGENOMiHOG000242438.
KOiK01556.
OMAiVWDLAHS.
OrthoDBiPOG091H0D63.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_01970. Kynureninase. 1 hit.
InterProiIPR000192. Aminotrans_V_dom.
IPR010111. Kynureninase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR14084. PTHR14084. 1 hit.
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF038800. KYNU. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01814. kynureninase. 1 hit.

Sequencei

Sequence statusi: Complete.

A5FMM4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTFQNTREFA KQLDAQDALN HYQEQFIFPK VNDKRVIYFT GNSLGLQPKR
60 70 80 90 100
TKAYIDEVMN DWAELAVEGH FYAEKPWWDY QERFSEPLSK IVGALPSEVT
110 120 130 140 150
VMNTLTVNLH LLMVSFYQPK GKRYKIICEE KAFPSDQYMF QSQVHFHGYK
160 170 180 190 200
PEDAIVEIKR REGEHNIRLE DVLAKIEEVG DELALVLIGG VNYYTGQVFD
210 220 230 240 250
IKTITAAGQK AGAKVGWDLA HAAGNIKLEL HDWNVDFAAW CSYKYMNSGP
260 270 280 290 300
GNASGVFVHE RHHNDPDLPR FAGWWGHNKE RRFKMEPNFD PVHGAGGWQI
310 320 330 340 350
SNLPVLSLAP YLASVEMFAE VGMDALIAKR DHITSYLEFI LHEIDKEVES
360 370 380 390 400
TFEIITPSNP EERASQLSVF LHGEGRSLFD YLMKNGVITD WREPNVIRLA
410 420
PVPLYCSYED MYDFGQILKK GILGK
Length:425
Mass (Da):48,720
Last modified:June 12, 2007 - v1
Checksum:i0070DB3521B3E741
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000685 Genomic DNA. Translation: ABQ03541.1.
RefSeqiWP_012022597.1. NC_009441.1.

Genome annotation databases

EnsemblBacteriaiABQ03541; ABQ03541; Fjoh_0506.
KEGGifjo:Fjoh_0506.
PATRICi21895273. VBIFlaJoh53613_0523.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000685 Genomic DNA. Translation: ABQ03541.1.
RefSeqiWP_012022597.1. NC_009441.1.

3D structure databases

ProteinModelPortaliA5FMM4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi376686.Fjoh_0506.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ03541; ABQ03541; Fjoh_0506.
KEGGifjo:Fjoh_0506.
PATRICi21895273. VBIFlaJoh53613_0523.

Phylogenomic databases

eggNOGiENOG4105CKY. Bacteria.
COG3844. LUCA.
HOGENOMiHOG000242438.
KOiK01556.
OMAiVWDLAHS.
OrthoDBiPOG091H0D63.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00329.
UPA00334; UER00455.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_01970. Kynureninase. 1 hit.
InterProiIPR000192. Aminotrans_V_dom.
IPR010111. Kynureninase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR14084. PTHR14084. 1 hit.
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF038800. KYNU. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01814. kynureninase. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKYNU_FLAJ1
AccessioniPrimary (citable) accession number: A5FMM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: June 12, 2007
Last modified: November 2, 2016
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.