A5FMJ5 (F16PA_FLAJ1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fructose-1,6-bisphosphatase class 1
Short name=FBPase class 1
EC=3.1.3.11

Alternative name(s):
D-fructose-1,6-bisphosphate 1-phosphohydrolase class 1

Gene names

Name:	fbp
Ordered Locus Names:	Fjoh_0533

Organism

Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / UW101) (Cytophaga johnsonae) [Complete proteome] [HAMAP]

Taxonomic identifier

376686 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Flavobacteriia › Flavobacteriales › Flavobacteriaceae › Flavobacterium

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate. HAMAP MF_01855
Cofactor	Binds 2 magnesium ions per subunit By similarity. HAMAP MF_01855
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_01855
Subunit structure	Homotetramer By similarity. HAMAP MF_01855
Subcellular location	Cytoplasm Potential HAMAP MF_01855.
Sequence similarities	Belongs to the FBPase class 1 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	fructose 1,6-bisphosphate 1-phosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	Fructose-1,6-bisphosphatase class 1 HAMAP MF_01855		PRO_0000364555
Regions
	Region	120 – 123	4	Substrate binding By similarity
Sites
	Metal binding	93	1	Magnesium 1 By similarity
	Metal binding	117	1	Magnesium 1 By similarity
	Metal binding	117	1	Magnesium 2 By similarity
	Metal binding	119	1	Magnesium 1; via carbonyl oxygen By similarity
	Metal binding	120	1	Magnesium 2 By similarity
	Metal binding	280	1	Magnesium 2 By similarity
	Binding site	213	1	Substrate By similarity
	Binding site	244	1	Substrate By similarity
	Binding site	274	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5FMJ5 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: A2A441DC9FEED8A2	FASTA	334	37,172
	10 20 30 40 50 60 MEERNKTLGE FIIENQKAFQ YSSGELSRII NSIRLAAKVV NYKVNKAGLV DIIGAAGEQN 70 80 90 100 110 120 IQGEDQQKLD VYANEVFIQT LINREIVCGI ASEENDDFIT VQGSDNCHNN KYVILMDPLD 130 140 150 160 170 180 GSSNIDVNVS VGTIFSVFRR ITPIGTPVTS EDFLQPGINQ VAAGYVIYGT STMLVYTTGF 190 200 210 220 230 240 GVNGFTLNPA IGTFYLSHPN MKFPENGNIY SVNEGNYVHF PQGVKNYIKY CQREEEDRPY 250 260 270 280 290 300 TSRYIGSLVA DFHRNMIKGG IYIYPTSSKA SKGKLRLLYE CNPMAFIAEQ AGGKATDGFG 310 320 330 RIMEIQPTEL HQRVPFFCGS KSMVEKAEEF MAAE

References

[1]

"Complete sequence of Flavobacterium johnsoniae UW101." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Goltsman E., Singan V., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Xie G., McBride M., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17061 / DSM 2064 / UW101.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000685 Genomic DNA. Translation: ABQ03568.1.
RefSeq	YP_001192887.1. NC_009441.1.
3D structure databases
ProteinModelPortal	A5FMJ5.
SMR	A5FMJ5. Positions 6-331.
ModBase	Search...
Protein-protein interaction databases
STRING	A5FMJ5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5089479.
GenomeReviews	Gene locus Fjoh_0533 in contig CP000685_GR.
KEGG	fjo:Fjoh_0533.
PATRIC	21895329. VBIFlaJoh53613_0551.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0158.
HOGENOM	HBG731261.
OMA	HWEAPVQ.
ProtClustDB	PRK09293.
Enzyme and pathway databases
BioCyc	FJOH376686:FJOH_0533-MONOMER.
Family and domain databases
HAMAP	MF_01855. FBPase_class1. [Tree]
InterPro	IPR000146. FBPase_class-1/SBPase. IPR020548. Fructose_bisphosphatase_AS. [Graphical view]
KO	K03841.
PANTHER	PTHR11556. In_FB_phphtase. 1 hit.
Pfam	PF00316. FBPase. 1 hit. [Graphical view]
PIRSF	PIRSF000904. FBPtase_SBPase. 1 hit.
PRINTS	PR00115. F16BPHPHTASE.
PROSITE	PS00124. FBPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F16PA_FLAJ1

Accession

Primary (citable) accession number: A5FMJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	June 12, 2007
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program