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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / UW101) (Cytophaga johnsonae)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei732 – 7321ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciFJOH376686:GIXN-584-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Fjoh_0565
OrganismiFlavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / UW101) (Cytophaga johnsonae)
Taxonomic identifieri376686 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesFlavobacteriiaFlavobacterialesFlavobacteriaceaeFlavobacterium
ProteomesiUP000006694 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 954954Leucine--tRNA ligasePRO_1000074833Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi376686.Fjoh_0565.

Structurei

3D structure databases

ProteinModelPortaliA5FMG2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 5112"HIGH" regionAdd
BLAST
Motifi729 – 7335"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5FMG2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKYNPNEIEA KWQKYWAENQ TFAAKNNSEK PKHYVLDMFP YPSGAGLHVG
60 70 80 90 100
HPLGYIASDV YSRFKRHQGF NVLHPMGYDS FGLPAEQYAI QTGQRPEDTT
110 120 130 140 150
RVNIDGGVDK EGKQIAGYRK QLDKIGFSFD WAREVRTSNP DYYKHTQWIF
160 170 180 190 200
IQLFNSWYCR KQGKAFDISE LVTVFEESGN ALVEAVCDDN VTIFTADEWK
210 220 230 240 250
SYSDDQKEKI LLQYRMTYLA ETEVNWCPGL GTVLANDEIV NGVSERGGFP
260 270 280 290 300
VIRKKMTQWS MRISAYAERL LQGLNDIDWS ESIKESQRNW IGKSVGALVT
310 320 330 340 350
FNVKNHDEVI EVFTTRPDTI FGVTFMTLAP EHDLVAKITT PEQKEAVEAY
360 370 380 390 400
IEKTAKRSER ERMADVKTIS GVFTGAYAEH PFTKEAIPVW IGDYVLAGYG
410 420 430 440 450
TGAVMAVPCG DERDYAFANF FKGQNGMQEI KNIFANVDIS EAAYGSKDNV
460 470 480 490 500
EIAASDFLNG LNYKDATAKA IYKLEEIGQG KGKTNYRLRD AVFSRQRYWG
510 520 530 540 550
EPFPVYYVNG LPKMIDTQHL PIILPEVEKY LPTEDGLPPL GNAAVWAWDI
560 570 580 590 600
KENKVVNTDL VDNVSIFPLE LNTMPGWAGS SWYWMRYMDA HNENEFASKE
610 620 630 640 650
ALAYWENVDL YIGGSEHATG HLLYSRFWNK FLKDKGFAPT EEPFKKLINQ
660 670 680 690 700
GMILGTTAYV YRLEGTNTFV SKNKIKGQNV QPIRVDVHFV NSSDELNIEK
710 720 730 740 750
FKAWREDFNT AEFIFDENGK YIVGREVEKM SKSYYNVVTP DDICAEYGAD
760 770 780 790 800
TLRLYEMFLG PLEQAKPWNT AGISGVFGFL KKLWRLYFDD NGLIVNNDEP
810 820 830 840 850
TKDNLKSLHK TIKKVAEDIE NFSFNTSVSQ FMICVNELSS QNCHSRAILE
860 870 880 890 900
PLAILVSPYA PHIAEELWAQ LGHKTSISEV AFPVFDAKHL VETNKEYPVS
910 920 930 940 950
FNGKMRFTIE LPLDLTAAQI EEIVMKDERT QKQLDGRIPN KVIIVPGKII

NLVG
Length:954
Mass (Da):108,655
Last modified:June 12, 2007 - v1
Checksum:iD29CBF67221F04B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000685 Genomic DNA. Translation: ABQ03600.1.
RefSeqiWP_012022656.1. NC_009441.1.
YP_001192919.1. NC_009441.1.

Genome annotation databases

EnsemblBacteriaiABQ03600; ABQ03600; Fjoh_0565.
KEGGifjo:Fjoh_0565.
PATRICi21895393. VBIFlaJoh53613_0583.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000685 Genomic DNA. Translation: ABQ03600.1.
RefSeqiWP_012022656.1. NC_009441.1.
YP_001192919.1. NC_009441.1.

3D structure databases

ProteinModelPortaliA5FMG2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi376686.Fjoh_0565.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ03600; ABQ03600; Fjoh_0565.
KEGGifjo:Fjoh_0565.
PATRICi21895393. VBIFlaJoh53613_0583.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciFJOH376686:GIXN-584-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis."
    McBride M.J., Xie G., Martens E.C., Lapidus A., Henrissat B., Rhodes R.G., Goltsman E., Wang W., Xu J., Hunnicutt D.W., Staroscik A.M., Hoover T.R., Cheng Y.Q., Stein J.L.
    Appl. Environ. Microbiol. 75:6864-6875(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 17061 / DSM 2064 / UW101.

Entry informationi

Entry nameiSYL_FLAJ1
AccessioniPrimary (citable) accession number: A5FMG2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: June 12, 2007
Last modified: April 29, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.