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A5FMG2 (SYL_FLAJ1) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Fjoh_0565
OrganismFlavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / UW101) (Cytophaga johnsonae) [Complete proteome] [HAMAP]
Taxonomic identifier376686 [NCBI]
Taxonomic lineageBacteriaBacteroidetesFlavobacteriiaFlavobacterialesFlavobacteriaceaeFlavobacterium

Protein attributes

Sequence length954 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 954954Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000074833

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif729 – 7335"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site7321ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A5FMG2 [UniParc].

Last modified June 12, 2007. Version 1.
Checksum: D29CBF67221F04B1

FASTA954108,655
        10         20         30         40         50         60 
MKYNPNEIEA KWQKYWAENQ TFAAKNNSEK PKHYVLDMFP YPSGAGLHVG HPLGYIASDV 

        70         80         90        100        110        120 
YSRFKRHQGF NVLHPMGYDS FGLPAEQYAI QTGQRPEDTT RVNIDGGVDK EGKQIAGYRK 

       130        140        150        160        170        180 
QLDKIGFSFD WAREVRTSNP DYYKHTQWIF IQLFNSWYCR KQGKAFDISE LVTVFEESGN 

       190        200        210        220        230        240 
ALVEAVCDDN VTIFTADEWK SYSDDQKEKI LLQYRMTYLA ETEVNWCPGL GTVLANDEIV 

       250        260        270        280        290        300 
NGVSERGGFP VIRKKMTQWS MRISAYAERL LQGLNDIDWS ESIKESQRNW IGKSVGALVT 

       310        320        330        340        350        360 
FNVKNHDEVI EVFTTRPDTI FGVTFMTLAP EHDLVAKITT PEQKEAVEAY IEKTAKRSER 

       370        380        390        400        410        420 
ERMADVKTIS GVFTGAYAEH PFTKEAIPVW IGDYVLAGYG TGAVMAVPCG DERDYAFANF 

       430        440        450        460        470        480 
FKGQNGMQEI KNIFANVDIS EAAYGSKDNV EIAASDFLNG LNYKDATAKA IYKLEEIGQG 

       490        500        510        520        530        540 
KGKTNYRLRD AVFSRQRYWG EPFPVYYVNG LPKMIDTQHL PIILPEVEKY LPTEDGLPPL 

       550        560        570        580        590        600 
GNAAVWAWDI KENKVVNTDL VDNVSIFPLE LNTMPGWAGS SWYWMRYMDA HNENEFASKE 

       610        620        630        640        650        660 
ALAYWENVDL YIGGSEHATG HLLYSRFWNK FLKDKGFAPT EEPFKKLINQ GMILGTTAYV 

       670        680        690        700        710        720 
YRLEGTNTFV SKNKIKGQNV QPIRVDVHFV NSSDELNIEK FKAWREDFNT AEFIFDENGK 

       730        740        750        760        770        780 
YIVGREVEKM SKSYYNVVTP DDICAEYGAD TLRLYEMFLG PLEQAKPWNT AGISGVFGFL 

       790        800        810        820        830        840 
KKLWRLYFDD NGLIVNNDEP TKDNLKSLHK TIKKVAEDIE NFSFNTSVSQ FMICVNELSS 

       850        860        870        880        890        900 
QNCHSRAILE PLAILVSPYA PHIAEELWAQ LGHKTSISEV AFPVFDAKHL VETNKEYPVS 

       910        920        930        940        950 
FNGKMRFTIE LPLDLTAAQI EEIVMKDERT QKQLDGRIPN KVIIVPGKII NLVG 

« Hide

References

[1]"Complete sequence of Flavobacterium johnsoniae UW101."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Goltsman E., Singan V., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Xie G., McBride M., Richardson P.
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 17061 / DSM 2064 / UW101.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000685 Genomic DNA. Translation: ABQ03600.1.
RefSeqYP_001192919.1. NC_009441.1.

3D structure databases

ProteinModelPortalA5FMG2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING376686.Fjoh_0565.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ03600; ABQ03600; Fjoh_0565.
GeneID5089511.
KEGGfjo:Fjoh_0565.
PATRIC21895393. VBIFlaJoh53613_0583.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycFJOH376686:GIXN-584-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_FLAJ1
AccessionPrimary (citable) accession number: A5FMG2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: June 12, 2007
Last modified: April 16, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries