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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / UW101) (Cytophaga johnsonae)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi491MagnesiumUniRule annotation1
Metal bindingi497MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Fjoh_1444
OrganismiFlavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / UW101) (Cytophaga johnsonae)
Taxonomic identifieri376686 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesFlavobacteriiaFlavobacterialesFlavobacteriaceaeFlavobacterium
Proteomesi
  • UP000006694 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003296471 – 714Polyribonucleotide nucleotidyltransferaseAdd BLAST714

Interactioni

Protein-protein interaction databases

STRINGi376686.Fjoh_1444.

Structurei

3D structure databases

ProteinModelPortaliA5FJZ1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini558 – 620KHUniRule annotationAdd BLAST63
Domaini630 – 701S1 motifUniRule annotationAdd BLAST72

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C62. Bacteria.
COG1185. LUCA.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiPOG091H00M0.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5FJZ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIPQVSQEII DLGDGRSISI ETGKLAKQAD GSVVVRLGNC MLLATAVSAR
60 70 80 90 100
TSNPGVDFLP LTVDYREKFA AAGRFPGGFF KREARPSDSE VLTMRLVDRV
110 120 130 140 150
LRPLFPDDYH AETQVMIQLM SHDDNVMPDA LAGLAASAAL AVSDIPFYNL
160 170 180 190 200
ISEVRVARID GKLVINPSRT ELEKSDIDMM IGASLDSVAM VEGEMKEISE
210 220 230 240 250
AEMIEAIKFA HEAIKVQIHA QQKLRAKLSS QEYRTYEGEV EDEAVYAKVK
260 270 280 290 300
AAAYDKCYAI AQEASGKAER GEKFATVKEE AKALFTEEEY AENADLAGLV
310 320 330 340 350
GKYFYKTNKE AVRNVILEKG IRLDGRKTTE IRPIWCETDY LPSVHGSSLF
360 370 380 390 400
TRGETQALAT VTLGTSREAN QIDSPSEQGE EKFYLHYNFP PFSTGEAKPL
410 420 430 440 450
RGTSRREVGH GNLAQRALKN MIPADCPYTI RVVSEVLESN GSSSMATVCA
460 470 480 490 500
GTMALMDAGV QMVKPVSGIA MGLITDGEKF AVLSDILGDE DHLGDMDFKV
510 520 530 540 550
TGTADGITAC QMDIKIDGLR YDIMEQALAQ ARDGRLHILG KLTETIAAPR
560 570 580 590 600
ADVKAHAPKI ITRTIPGNFI GALIGPGGKV IQELQKATGT TIVINEVDEQ
610 620 630 640 650
GVVEILGTDP EGIKTVLAKI DALTFKPQVG EAYEVKVIKM LDFGAVVEYT
660 670 680 690 700
AAPGNEVLLH VSELAWERTE NVADVVKMGD VFEVKYLGVD PKTKKEKVSK
710
KALVPRPPRE DKKE
Length:714
Mass (Da):77,853
Last modified:June 12, 2007 - v1
Checksum:i933BD9396FD6EA24
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000685 Genomic DNA. Translation: ABQ04476.1.
RefSeqiWP_012023523.1. NC_009441.1.

Genome annotation databases

EnsemblBacteriaiABQ04476; ABQ04476; Fjoh_1444.
KEGGifjo:Fjoh_1444.
PATRICi21897219. VBIFlaJoh53613_1485.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000685 Genomic DNA. Translation: ABQ04476.1.
RefSeqiWP_012023523.1. NC_009441.1.

3D structure databases

ProteinModelPortaliA5FJZ1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi376686.Fjoh_1444.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ04476; ABQ04476; Fjoh_1444.
KEGGifjo:Fjoh_1444.
PATRICi21897219. VBIFlaJoh53613_1485.

Phylogenomic databases

eggNOGiENOG4105C62. Bacteria.
COG1185. LUCA.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiPOG091H00M0.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPNP_FLAJ1
AccessioniPrimary (citable) accession number: A5FJZ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: June 12, 2007
Last modified: November 2, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.