A5FJR8 (METE_FLAJ1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	Fjoh_1519

Organism

Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / UW101) (Cytophaga johnsonae) [Complete proteome] [HAMAP]

Taxonomic identifier

376686 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Flavobacteriia › Flavobacteriales › Flavobacteriaceae › Flavobacterium

Protein attributes

Sequence length	774 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 774	774	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000097829
Sites
	Metal binding	655	1	Zinc By similarity
	Metal binding	657	1	Zinc By similarity
	Metal binding	740	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5FJR8 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 01FA76C75FC40C77	FASTA	774	87,929
	10 20 30 40 50 60 MKTNNLGYPR IGSNRELKKA SELYWAGKIS ADELLDAGKE IRLKNWYLQS EAGVDLIPSN 70 80 90 100 110 120 DFSFYDQVLD LTLAVGAIPQ RYHELAKSNS SLDLYFAMAR GSQKNGQDVV AMEMTKWFDT 130 140 150 160 170 180 NYHYIVPEFT KNQKFELFSE KILNEFKEAN AVGIKTKPVL IGPVSYLLLG KEKEEGFNRI 190 200 210 220 230 240 DLIDALLPVY FEILEKLQIE NAEYIQLDEP FLALNLTDKE RNVFTKVYNE INVRFPKLKI 250 260 270 280 290 300 VLANYFDCFG ENLETALALP VDTFHLDLVR CQLQLDDILE SGKLASNVNL SLGVVDGRNI 310 320 330 340 350 360 WKNDFKKSLE LIKKAADALG ENRILLAPSC SLIHSPCDLD LETNDQTLTP EIKQWLAFAK 370 380 390 400 410 420 QKINEVVLLK QFASGEVNPK NSADYERNVI ANENRKTSKL IHNNEVKART AGITAADDKR 430 440 450 460 470 480 KSAFASRRKS QIKALNLPLF PTTTIGSFPQ TTEVRSWRAK FKKGELTTEQ YNDLIEKETE 490 500 510 520 530 540 AAIRFQEETG IDVLVHGEFE RNDMVEYFGE QLDGFTFTKN GWVQSYGSRC VKPPVIYGDV 550 560 570 580 590 600 SRPNPMTVKW SKYAQSLTSK WVKGMLTGPV TILQWSFVRN DQPRSETCTQ IALAVRDEVV 610 620 630 640 650 660 DLEKAGIKII QIDEPAIREG LPLRKEEWAK YLDWAVKAFR ISASGVNDDT QIHPHMCYSE 670 680 690 700 710 720 FNDIIQNIAD MDADVITIEC SRSQMELLDA FANFKYPNEI GPGVYDIHSP RVPSSKEMVR 730 740 750 760 770 LLEKASAVIP VDQLWVNPDC GLKTRHWNET KKALIEMVAA AQEMRKAVEN PVTE

References

[1]

"Complete sequence of Flavobacterium johnsoniae UW101." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Goltsman E., Singan V., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Xie G., McBride M., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17061 / DSM 2064 / UW101.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000685 Genomic DNA. Translation: ABQ04551.1.
RefSeq	YP_001193870.1. NC_009441.1.
3D structure databases
ProteinModelPortal	A5FJR8.
SMR	A5FJR8. Positions 3-766.
ModBase	Search...
Protein-protein interaction databases
STRING	A5FJR8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5091265.
GenomeReviews	Gene locus Fjoh_1519 in contig CP000685_GR.
KEGG	fjo:Fjoh_1519.
PATRIC	21897379. VBIFlaJoh53613_1565.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0620.
HOGENOM	HBG287495.
OMA	RNIWRAN.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	FJOH376686:FJOH_1519-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_FLAJ1

Accession

Primary (citable) accession number: A5FJR8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 12, 2007
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program