A5FHX5 (RLMF_FLAJ1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase F
EC=2.1.1.181

Alternative name(s):
23S rRNA mA1618 methyltransferase
rRNA adenine N-6-methyltransferase

Gene names

Name:	rlmF
Ordered Locus Names:	Fjoh_2174

Organism

Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / UW101) (Cytophaga johnsonae) [Complete proteome] [HAMAP]

Taxonomic identifier

376686 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Flavobacteriia › Flavobacteriales › Flavobacteriaceae › Flavobacterium

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the adenine in position 1618 of 23S rRNA By similarity. HAMAP MF_01848
Catalytic activity	S-adenosyl-L-methionine + adenine(1618) in 23S rRNA = S-adenosyl-L-homocysteine + rRNA containing N(6)-methyladenine(1618) in 23S rRNA. HAMAP MF_01848
Subcellular location	Cytoplasm Potential HAMAP MF_01848.
Sequence similarities	Belongs to the methyltransferase superfamily. METTL16/rlmF family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA (adenine-N6-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	Ribosomal RNA large subunit methyltransferase F HAMAP MF_01848		PRO_0000349916

Sequences

	Sequence		Length	Mass (Da)
	A5FHX5 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 60D45F1506CDF5C8	FASTA	316	35,867
	10 20 30 40 50 60 MKAENNSQKD NLHPRNLHRS RYDFELLISN CPELKAFISI NKHGIETVDF SNPLAVKTLN 70 80 90 100 110 120 KALLQTYYDI QNWDIPKNYL CPPIPGRADY IHYLADLLAE TNNGKIPEGN SVMGLDIGTG 130 140 150 160 170 180 ANLIYPILGN SIYNWSFVAT DIYKTSIENA SKIIEANPRL IDAVSLQQQT EPRFIFKNII 190 200 210 220 230 240 IPEDRFTFTM CNPPFHASAE EANKSTSRKV SNLNPKEKKN TNPVLNFGGQ NAELWCNGGE 250 260 270 280 290 300 IGFITQMIYE SVKYASQVLW FTTLVSKKEN LSSIYKTLKK VNAVSVKTIE MSQGQKNSRI 310 VAWSFLNNTE QKSWKF

References

[1]

"Complete sequence of Flavobacterium johnsoniae UW101." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Goltsman E., Singan V., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Xie G., McBride M., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17061 / DSM 2064 / UW101.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000685 Genomic DNA. Translation: ABQ05202.1.
RefSeq	YP_001194521.1. NC_009441.1.
3D structure databases
ProteinModelPortal	A5FHX5.
ModBase	Search...
Protein-protein interaction databases
STRING	A5FHX5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5092793.
GenomeReviews	Gene locus Fjoh_2174 in contig CP000685_GR.
KEGG	fjo:Fjoh_2174.
PATRIC	21898779. VBIFlaJoh53613_2241.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3129.
HOGENOM	HBG747880.
OMA	DFANPEA.
ProtClustDB	PRK11727.
Enzyme and pathway databases
BioCyc	FJOH376686:FJOH_2174-MONOMER.
Family and domain databases
HAMAP	MF_01848. 23SrRNA_methyltr_F. [Tree]
InterPro	IPR016909. rRNA_lsu_methyltransferase_F. IPR010286. SAM-Mtase_prd. [Graphical view]
KO	K06970.
PANTHER	PTHR13393. DUF890. 1 hit.
Pfam	PF05971. Methyltransf_10. 1 hit. [Graphical view]
PIRSF	PIRSF029038. Mtase_YbiN_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMF_FLAJ1

Accession

Primary (citable) accession number: A5FHX5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	June 12, 2007
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program