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A5FHP3 (MDH_FLAJ1) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate dehydrogenase

EC=1.1.1.37
Gene names
Name:mdh
Ordered Locus Names:Fjoh_2255
OrganismFlavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / UW101) (Cytophaga johnsonae) [Complete proteome] [HAMAP]
Taxonomic identifier376686 [NCBI]
Taxonomic lineageBacteriaBacteroidetesFlavobacteriiaFlavobacterialesFlavobacteriaceaeFlavobacterium

Protein attributes

Sequence length311 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_00487

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_00487

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcellular carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

malate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 311311Malate dehydrogenase HAMAP-Rule MF_00487
PRO_1000081360

Regions

Nucleotide binding7 – 126NAD By similarity
Nucleotide binding118 – 1203NAD By similarity

Sites

Active site1751Proton acceptor By similarity
Binding site321NAD By similarity
Binding site821Substrate By similarity
Binding site881Substrate By similarity
Binding site951NAD By similarity
Binding site1201Substrate By similarity
Binding site1511Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
A5FHP3 [UniParc].

Last modified June 12, 2007. Version 1.
Checksum: C227E247EEB41128

FASTA31132,562
        10         20         30         40         50         60 
MKVTIVGAGN VGATCADVIS YRGIASEVVL LDIKEGFAEG KALDITQCAT NTGFNTKVSG 

        70         80         90        100        110        120 
VTNDYSKTAG SDVVVITSGI PRKPGMTREE LIGINAGIVK TVAENVLKYS PNTIIVVVSN 

       130        140        150        160        170        180 
PMDTMTYLAL KATGLPKNRI IGMGGALDSS RFRTYLSLAL DKPANDISAM VIGGHGDTTM 

       190        200        210        220        230        240 
IPLTRLASYN GIPVSQFLSE DVLQKVAADT MVGGATLTGL LGTSAWYAPG ASVAYLVDSI 

       250        260        270        280        290        300 
LNDQKKMIAC SVFVEGEYGQ NDICIGVPCI IGKNGVEEIV NINLNDQEKA LFAKSADAVR 

       310 
GMNDALKSIL V 

« Hide

References

[1]"Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis."
McBride M.J., Xie G., Martens E.C., Lapidus A., Henrissat B., Rhodes R.G., Goltsman E., Wang W., Xu J., Hunnicutt D.W., Staroscik A.M., Hoover T.R., Cheng Y.Q., Stein J.L.
Appl. Environ. Microbiol. 75:6864-6875(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 17061 / DSM 2064 / UW101.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000685 Genomic DNA. Translation: ABQ05283.1.
RefSeqYP_001194602.1. NC_009441.1.

3D structure databases

ProteinModelPortalA5FHP3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING376686.Fjoh_2255.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ05283; ABQ05283; Fjoh_2255.
GeneID5092261.
KEGGfjo:Fjoh_2255.
PATRIC21898941. VBIFlaJoh53613_2322.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0039.
HOGENOMHOG000213794.
KOK00024.
OMAYGQNDIC.
OrthoDBEOG6091FG.

Enzyme and pathway databases

BioCycFJOH376686:GIXN-2312-MONOMER.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPMF_00487. Malate_dehydrog_3.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11540. PTHR11540. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSPR00086. LLDHDRGNASE.
SUPFAMSSF56327. SSF56327. 1 hit.
TIGRFAMsTIGR01763. MalateDH_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMDH_FLAJ1
AccessionPrimary (citable) accession number: A5FHP3
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: June 12, 2007
Last modified: May 14, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families