A5FH28 (SERC_FLAJ1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoserine aminotransferase
EC=2.6.1.52

Alternative name(s):
Phosphohydroxythreonine aminotransferase
Short name=PSAT

Gene names

Name:	serC
Ordered Locus Names:	Fjoh_2462

Organism

Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / UW101) (Cytophaga johnsonae) [Complete proteome] [HAMAP]

Taxonomic identifier

376686 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Flavobacteriia › Flavobacteriales › Flavobacteriaceae › Flavobacterium

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine By similarity. HAMAP MF_00160
Catalytic activity	O-phospho-L-serine + 2-oxoglutarate = 3-phosphonooxypyruvate + L-glutamate. HAMAP MF_00160 4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate. HAMAP MF_00160
Cofactor	Binds 1 pyridoxal phosphate per subunit By similarity. HAMAP MF_00160
Pathway	Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 2/3. HAMAP MF_00160 Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 3/5. HAMAP MF_00160
Subunit structure	Homodimer By similarity. HAMAP MF_00160
Subcellular location	Cytoplasm By similarity HAMAP MF_00160.
Sequence similarities	Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Pyridoxine biosynthesis Serine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW pyridoxine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	O-phospho-L-serine:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	Phosphoserine aminotransferase HAMAP MF_00160		PRO_1000076906
Regions
	Region	76 – 77	2	Pyridoxal phosphate binding By similarity
	Region	234 – 235	2	Pyridoxal phosphate binding By similarity
Sites
	Binding site	41	1	L-glutamate By similarity
	Binding site	102	1	Pyridoxal phosphate By similarity
	Binding site	150	1	Pyridoxal phosphate By similarity
	Binding site	169	1	Pyridoxal phosphate By similarity
	Binding site	192	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	193	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5FH28 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 001013680A26430E	FASTA	356	38,871
	10 20 30 40 50 60 MKKHNYSAGP SILPQEVFEK ASKAVLNFND SGLSILEISH RSKDFVAVMD EARSLALELL 70 80 90 100 110 120 GLQGKGYQAL FLQGGASTAF LMAPYNLMKE NGKAAYLDSG TWATAAIKEA KLFGETVIVG 130 140 150 160 170 180 SSKDDNYTYI PKGYEIPADA DYFHCTSNNT IFGTQIQEFP STNIPVVCDM SSDIFSRELD 190 200 210 220 230 240 FSKFDLIYAG AQKNMGPAGT TLVVVKEEIL GKNGRTIPSM LDYAKHIKAE SMYNTPSVFA 250 260 270 280 290 300 VYVSLLTLQW IKAKGGIAAV EKLNNAKADL LYAEIDRNPL FKGAANVEDR SKMNVTFLLN 310 320 330 340 350 NPEHTETFDA LWKAAGISGL PGHRSVGGYR ASIYNAMPIE SVQVLVDVMK ALESKV

References

[1]

"Complete sequence of Flavobacterium johnsoniae UW101." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Goltsman E., Singan V., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Xie G., McBride M., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17061 / DSM 2064 / UW101.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000685 Genomic DNA. Translation: ABQ05489.1.
RefSeq	YP_001194808.1. NC_009441.1.
3D structure databases
ProteinModelPortal	A5FH28.
ModBase	Search...
Protein-protein interaction databases
STRING	A5FH28.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5090319.
GenomeReviews	Gene locus Fjoh_2462 in contig CP000685_GR.
KEGG	fjo:Fjoh_2462.
PATRIC	21899361. VBIFlaJoh53613_2532.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1932.
HOGENOM	HBG289982.
OMA	YEVLFLQ.
ProtClustDB	PRK05355.
Enzyme and pathway databases
BioCyc	FJOH376686:FJOH_2462-MONOMER.
Family and domain databases
HAMAP	MF_00160. SerC_aminotrans_5. [Tree]
InterPro	IPR000192. Aminotrans_V/Cys_dSase. IPR020578. Aminotrans_V_PyrdxlP_BS. IPR022278. Pser_aminoTfrase. IPR003248. Pser_aminoTfrase_subgr. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KO	K00831.
PANTHER	PTHR21152:SF1. PTHR21152:SF1. 1 hit.
Pfam	PF00266. Aminotran_5. 1 hit. [Graphical view]
PIRSF	PIRSF000525. SerC. 1 hit.
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITE	PS00595. AA_TRANSFER_CLASS_5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SERC_FLAJ1

Accession

Primary (citable) accession number: A5FH28

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	June 12, 2007
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program